补肾活血方中药对妊娠大鼠子宫内膜容受性的影响  

The Effect of Bushenhuoxue Recipe on Endometrial Receptivity in Pregnancy Mice

作　　者：宋殿荣[1] 刘亚琴[1] 张崴[1] 李沛霖[1] 

机构地区：[1]天津中医药大学第二附属医院妇科,300150

出　　处：《国际妇产科学杂志》2009年第2期161-163,166,共4页Journal of International Obstetrics and Gynecology

基　　金：天津市高等学校科技发展基金项目(20050301)

摘　　要：目的:观察补肾活血方中药对妊娠大鼠子宫内膜容受性的影响。方法:选用妊娠SD大鼠随机分为空白组、模型组和中药组,于妊娠第1天模型组和中药组大鼠皮下注射米非司酮溶液1次(米非司酮溶液5g/L,5mg/kg)造成胚泡着床障碍模型,空白组皮下注射生理盐水1次(1mL/kg)。空白组和模型组从妊娠第1天起予生理盐水灌胃(6.7mL/kg),2次/d,连续5d。中药组从妊娠第1天起行补肾活血方中药灌胃(7.6g生药/kg),2次/d,连续5d。于妊娠第5天扫描电镜观察各组子宫内膜胞饮突发育情况,逆转录聚合酶链反应(RT-PCR)检测子宫内膜整合素αvβ3 mRNA的表达,于妊娠第10天计数各组大鼠胚泡着床数。结果:模型组子宫内膜表面未见明显的胞饮突发育,表现为增生早期的子宫内膜变化,中药组可见发育中的胞饮突,发育形态明显超前于模型组,接近于空白组;模型组αvβ3 mRNA表达水平(0.8545±0.0747,0.3984±0.0937)显著低于空白组(1.0518±0.0878,0.6016±0.1338)(P<0.01),中药组αvβ3 mRNA表达水平(0.9876±0.0751,0.5396±0.0584)显著高于模型组(P<0.05);模型组胚泡着床数(4.63±3.66)显著低于空白组(12.63±2.07)(P<0.01),中药组胚泡着床数(10.75±2.44)显著高于模型组(P<0.01)。结论:补肾活血方中药能明显改善胚泡着床障碍大鼠子宫内膜表面胞饮突的发育,并显著提高子宫内膜整合素αvβ3mRNA的表达,有助于子宫内膜容受性的建立,从而最终提高胚泡的着床率。Objective： To observe the effect of Bushenhuoxue recipe on endometrial receptivity in pregnancy rat. Methods： SD pregnancy rats were randomly assigned to control group, model group and treatment group. Rat were injected with mifepristone subcutaneously on the fast day of pregnancy （Pdl） to induce blastocyst implantation dysfunctional model in both model group and treatment group, the control group injected with saline as instead. Rat in treatment group were given the bushenhuoxue recipe decoction twice a day orally from Pdl to Pd5, while rats in model group and control group were administered saline instead. On Pd5, development of endometrial pinopodes in the rat uterus was determined by scanning electron microscopy in each group, and expression of integrin αvβ3 was examined by reverse transcript polymerase chain reaction （RT-PCR）. On Pd10, the numbers of implanted blastocyst was counted in each group. Results： In model group, no developed pinopodes of endometrial surface was found, the endometrium is in early proliferative phase. Developed pinopodes were obversed in both treatment and control group. The exepression of integrin αvβ3 mRNA in model group （0.854 5±0.074 7,0.398 4±0.093 7） were significantly lower than control group （1.051 8±0.087 8,0.601 6±0.133 8） （P 〈0.01） . The expression of αvβ3 mRNA in treatment group （0.9876±0.0751,0.5396±0.0584） were higher than model group, there were significantly differences between two groups （P 〈0.05 ）.The number of implanted blastocysts wer （4.63 ±3.66）in model group ,it was significantly lower than that of control group （12.63±2.07） （P 〈0.01 ） , and treatment group （10.75±2.44） （P 〈 0.01 ）. Conclusion： Bushenhuoxue recipe could improve pinopodes development of endometrial surface in blastocyst implantationin dysfunctional rat, and increase expression of integrin αvβ3 mRNA. It also improve uterine receptivity and increase the implantation of blastocyst.

关 键 词：子宫内膜容受性 胞饮突 整合素 补肾活血方 

分 类 号：R285.5[医药卫生—中药学]