聚L-半胱氨酸/多壁碳纳米管复合修饰电极同时测定邻苯二酚和对苯二酚  

Simultaneous Determination of Catechol and Hydroquinone at Poly-L-Cys/MultiWall Carbon Nanotubes Composite Film Modified Glassy Carbon Electrode

作　　者：余芬[1] 万其进[1] 朱丽娜[1] 邹菁[1] 喻德忠[1] 艾军[1] 

机构地区：[1]武汉工程大学化工与制药学院绿色化学过程省部共建教育部重点试验室,武汉430073

出　　处：《理化检验（化学分册）》2009年第4期375-379,383,共6页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：湖北省教育厅科学研究基金资助(D20091501)

摘　　要：将羧基化多壁碳纳米管分散在L-半胱氨酸溶液中并滴涂在玻碳电极表面。将上述电极在pH6．9的B-R缓冲溶液中，于-1.0～2．5V的电位范围内进行电聚合，制备了聚L-半胱氨酸／多壁碳纳米管复合修饰电极（Poly-L-Cys／MWCNTs／GCE）。研究发现，邻苯二酚和对苯-2-酚在聚L-半胱氨酸／多壁碳纳米管复合修饰电极上分别出现了一对氧化还原峰，且两者的氧化峰电位差达101mV，提出了用微分脉冲伏安法同时测定邻苯二酚和对苯二酚的方法。氧化峰电流与邻苯二酚和对苯二酚的浓度在1．0×10^-5～1．0×10^-3mol·L^-1呈线性关系，检出限（3S／N）均达1．0×10^-6mol·L^-1。修饰电极用于模拟样品中邻苯二酚和对苯二酚的测定，回收率在82．0％～107．0％之间。Functionalized multi-wall nano-carbon tubes （MWNT＇s） was dispersed in L-cysteine solution. The mixed solution obtained was dropped on the surface of glassy carbon electrode （GCE）, and then polymerised electrochemically in pH 6.9 B-R buffer solution at the potential of -1.0-2.5V. This modified electrode prepared as described above was named abbreviately as Poly-L-Cys/MWCNTs/GCE. It was found that either of catechol and hydroquinone showed a pair of oxidation peak and reduction peak at Poly-L-Cys/MWCNTs/GCE, with potential difference of the oxidation peak of both compounds attained to the value of 101mV. Based on these findings, a selectrive method for simultaneous determination of eatechol and hydroquinone by differential pulse voltammetry was proposed. Linear relationships between values of peak current and concentration of catechol and hydroquinone were obtained in the range of 1.0×10^-5--1.0×10^-3 mol·L^-1 , with their detection limits （3S/N） of 1.0×19^-6 mol·L^-1. The proposed method was used in the analysis of simulated sample, giving values of recovery of catechol and hydroquinone in the range of 82. 0%-107. 0%.

关 键 词：聚L-半胱氨酸 多壁碳纳米管 复合膜修饰电极 邻苯二酚 对苯二酚 

分 类 号：O657.1[理学—分析化学]