盐酸吡格列酮-乙基曙红荷移光度测定法  

Spectrophotometric Determination of Pioglitazone Hydrochloride by Its Charge-Transfer Reaction with Ethyl Eosin

作　　者：秦宗会[1] 

机构地区：[1]长江师范学院化学及环境科学系,重庆408100

出　　处：《理化检验（化学分册）》2009年第4期390-393,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：重庆市教委科委研(KJ081301);重庆市涪陵区科委科技计划(2008-43);重庆市高等学校优秀中青年骨干教师资助计划[渝教人(2005)2号];长江师范学院科研(07kg50)资助项目

摘　　要：在pH4．0的盐酸-乙酸钠缓冲介质中，吡格列酮（PGTZ）与乙基曙红（EE）之间发生荷移反应，形成离子缔合物，使溶液发生褪色现象，且在516nm波长处测定时，溶液的褪色程度与盐酸吡格列酮的浓度在9．75×10^-7～1．26×10^-5mol·L^-1范围内呈线性关系。表观摩尔吸光率（ε516）为6．44×10^4L·mol^-1·cm^-1，检出限（3s/k）为3．25×10^-7mol．L^-1。方法用于市售药品中PGTZ含量的测定，所得结果与标示值及高效液相色谱法（HPLC）测得结果相符。以血浆及尿液样品为基体加入标准溶液作回收试验，测得其回收率在97．7％～103．3％之间，测定值的相对标准偏差（n=6）均小于2．5％。In an acetate buffer medium of pH 4. 0, due to the charge transfer reaction, an ion association complex was formed between pioglitazone （PGTZ） and ethyl eosin （EE）, leading to color-fading of EE. Linear relationship between the magnitude of decrease in absorbance and the concentration of PGTZ was kept in the range of 9. 75×10^-7--1. 26×10^-5 mol·L^-1 when measured at the wavelength of 516 nm. The apparent molar absorptivity （ε516） found was 6. 44×10^4L·mol^-1·cm^-1, and detection limit （3s/k） of the method was found to be 3.25×10^-7 mol·L^-1. Samples of tablets and capsules of pioglitazone hydrochloride were analyzed by the proposed method, and the amounts of PGTZ found were checked quite well with the labelled values and also with the results obtained by HPLC. Recovery and precision of the method were tested by the standard addition method using samples of human blood serum and urine as matrixes, values of recovery found were in the range of 97. 7%--103.3%, and values of RSD＇s （n=6） found were less than 2. 5%.

关 键 词：分光光度法 荷移反应 盐酸吡格列酮 乙基曙红 

分 类 号：O657.32[理学—分析化学]