rAAV-CD151转染心肌梗死小型猪增加缺血心肌血流灌注  

rAAV-CD151 promotes myocardial blood perfusion in a swine ischemia model

作　　者：刘晓春[1] 左后娟[2] 刘正湘[2] 曾和松[2] 刘涛[2] 文莎[2] 汪道文[2] 张欣[3] 

机构地区：[1]华中科技大学同济医学院神经生物学系,武汉430030 [2]华中科技大学同济医学院附属同济医院心内科 [3]美国田纳西大学分子科学部

出　　处：《临床心血管病杂志》2009年第4期290-293,共4页Journal of Clinical Cardiology

基　　金：国家自然科学基金项目资助(No.30572728No.30670856)

摘　　要：目的:观察CD151重组腺相关病毒转染心肌梗死小型猪模型对缺血心肌血流灌注的影响,以明确CD151在体内血运重建中的作用。方法:结扎小型猪左前降支建立心肌梗死动物模型。包装CD151、antiCD151和GFP重组腺相关病毒,分点注射至梗死心肌及周围心肌进行基因转染。8周后13N-NH3PET评价心肌血流灌注。结果:CD151基因转染促进心肌组织局部CD151表达增高,明显促进缺血心肌血流灌注。rAAV-CD151组灌注图像心肌缺血总分值为10.82±2.36,明显小于rAAV-GFP组19.33±1.67(P<0.01),而rAAV-an-tiCD151组缺损总分值25.18±2.73,明显大于rAAV-GFP组和rAAV-CD151组(P<0.01)。结论:CD151在体内具有明显的促血管生成作用,能明显促进心肌梗死后血运重建并增加缺血心肌血流灌注。Objective：To investigate whether CD151 gene delivery promotes blood perfusion after myocardial infarction in swines. Method：Swines receivied coronary artery ligation and intramuscularly＇injection with rAAV CD151, rAAV-antiCD151 and rAAV GFP. Eight weeks after coronary artery ligation, the expression of CD151 was measured. ^13 N-labeled NH3 PET was done to evaluate blood perfusion. Result：Compared to the control group and the rAAV-GFP group, the rAAV CD151 group show higher CD151 protein expression, whereas the rAAV- antiCD151 group showed reversed changes. 13N NH3 PET imaging showed that the blood perfusion was improved and the myocardial ischemia scores were （[10.82±2.36] vs [-（19.33±1.67],P〈0.01） in rAAV CD151 groupcompared with rAAV-GFP group. The myocardial ischemia score in the rAAV antiCD151 group was 25.18 ± 2.73, much larger than rAAV CD151 group （P%0.01）. Conclusion： rAAV CD151 direct injection can transfect the myocardium and express the CD151 protein. This study suggested that CD151 could promote neovascularization and blood perfusion.

关 键 词：心肌梗死 血管生成 CD151 基因转染 

分 类 号：R541.1[医药卫生—心血管疾病]