苯并(a)芘作用下人支气管上皮细胞蛋白表达谱的改变  

Differentially Expressed Proteins of Human Bronchial Epithelial Cells Exposed to Benzo(a)pyrene

作　　者：杨瑾[1] 刘晓勇[1] 段燕英[1] 牛丕业[1] 邬堂春[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院教育部环境与健康重点实验室,湖北武汉430030

出　　处：《环境与职业医学》2009年第2期110-114,共5页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助(编号:30525031);国家重点基础研究规划项目课题(编号:2002CB512905)

摘　　要：[目的]观察苯并(a)芘(BaP)作用下人支气管上皮细胞(16HBE)蛋白表达谱的改变。[方法]以0、1、2、4、8、16、32、64μmol/L的BaP染毒细胞24h,应用双向凝胶电泳和ImageMaster软件分析BaP处理后细胞内蛋白质表达谱的改变,基质辅助激光解吸附电离-飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF)结合数据库检索鉴定差异表达蛋白质斑点。[结果]各浓度染毒组与对照组相比,成功鉴定了17个差异表达蛋白质斑点,其中表达上调的有9种,下调的有8种。这些蛋白质包括细胞骨架蛋白、与能量代谢有关的蛋白、与DNA损伤修复有关的蛋白、肿瘤标志物、伴侣蛋白、与信号转导有关的蛋白和与氧化应激有关的蛋白。[结论]BaP作用于16HBE后导致与细胞损伤、DNA修复、应激、细胞恶性转化等有关的蛋白质表达发生改变。[ Objective ] To investigate the differentially expressed proteins of human bronchial epithelial cells （ 16HBE ） exposed to benzo[a]pyrene （BaP）. [ Methods ] The 16HBE cells were exposed to 0, 1, 2, 4, 8, 16, 32 and 64μmol/L BaP for 24 h. Two-dimensional electrophoresis was used to investigated the levels of proteins in 16HBE exposed to BaP and matrix-assisted laser desorption/ionization time of flight mass spectrometry combined with database were applied to identify the differentially expressed proteins. [ Results ] The expressions of proteins were changed after cells exposed to BaP, and then 9 increased proteins and 8 decreased proteins were successfully identified, including cytoskeleton proteins, energy metabolism proteins, DNA damage recognition and repair proteins, tumor biomarker, chaperone proteins, signal transduction proteins and oxidative stress proteins. [ Conclusion ] The expression spectrum of proteins in 16HBE exposed to BaP had been changed, and the identified proteins involved in cell structure, energy metabolism, DNA damage recognition and repair, cancer, signal transduction and chaperones.

关 键 词：苯并(A)芘 双向电泳 人支气管上皮细胞 

分 类 号：R114[医药卫生—卫生毒理学]