Fe_2O_3纳米颗粒对人肝癌细胞游离钙的影响  

Effects of Fe_2O_3 Nanoparticles on Free Calcium in Human Hepatoma Cells

作　　者：陆敏玉[1] 唐萌[1] 夏婷[1] 殷海荣[1] 杨扬[1] 王姝[1] 张婷[1] 孔璐[1] 张宇[1] 顾宁[1] 薛玉英[1] 

机构地区：[1]东南大学公共卫生学院,江苏南京210009

出　　处：《环境与职业医学》2009年第2期133-135,共3页Journal of Environmental and Occupational Medicine

基　　金：国家自然基金资助项目(编号:30671782);国家"973"计划资助项目(编号:2006CB705602)

摘　　要：[目的]探讨三氧化二铁(Fe2O3)纳米颗粒对人肝癌细胞(HepG2细胞)形态学和游离钙([Ca2+]i)的影响,为研究Fe2O3纳米颗粒诱导细胞凋亡的机制提供依据。[方法]以不同浓度(0.173、0.347、0.694g/L)的Fe2O3纳米颗粒对HepG2细胞染毒1、12、24h,用激光扫描共聚焦显微镜检测HepG2细胞[Ca2+]i荧光强度的变化。[结果]与对照组相比,染毒不同时间各染毒组细胞内[Ca2+]i含量差异均有统计学意义(P<0.05);染毒不同浓度时,Fe2O3纳米颗粒均能不同程度地引起HepG2细胞的形态学改变及其细胞内[Ca2+]i的升高。0.347、0.694g/L组染毒不同时间点的平均荧光强度分别为127.33±20.13、108.88±19.15、144.00±13.86和119.67±1.15、122.35±11.47、186.40±17.34,均明显高于对照组54.33±6.43,差异有统计学意义(P<0.05)。[结论]HepG2细胞凋亡可能与Fe2O3纳米颗粒引起的细胞[Ca2+]i升高有关。[ Objective ] To study the effects of iron sesquioxide nanoparticles （ nano-Fe2O3） on morphology and free calcium（[Ca^2＋]i）concentration in human hepatoma cell line（ HepG2 cell ）. To provide evidence for the mechanism of nano-Fe203- induced apoptosis in HepG2 cell. [ Methods ] HepG2 cells were exposed to nano-Fe203 of different concentrations （0.173, 0.347, and 0.694 g/L ）for 1, 12, and 24h. Laser scanning confocal microscopy was used to detect the fluorescence intensity of [ Ca^2＋ ]i in HepG2 cells. [ Results ] Compared with the control group, treated for different time duration, differences of intracellular[ Ca^2＋ ]i concentration between dose groups were significant（ P 〈 0.05 ）. The morphology was changed and the[ Ca^2＋ ]i concentration increased in HepG2 celt after exposure to different concentrations of nano-Fe2O3. Intracellular[ Ca^2＋ ] i concentrations at 1, 12, and 24h exposure to 0.347, and 0.694g/L nano-Fe2O3 were 127.33±20.13、108.88±19.15、144.00±13.86, and 119.67±1.15、122.35±11.47、186.40±17.34 respectively, and all higher than control group （54.33±6.43 ）（P 〈 0.05 ）. [Conclution] HepG2 apoptosis might be related to the increase of the intracellular [Ca^2＋]i level caused by nano-Fe2O3.

关 键 词：激光扫描共聚焦显微镜 游离钙 三氧化二铁 纳米颗粒 人肝癌细胞株 细胞毒性 

分 类 号：R114[医药卫生—卫生毒理学]