癌性胸水脱落细胞端粒酶活性表达初步研究  

Telomerase Activity Expressed Study in Exfoliated Cells of Cancer Chest Fluid

作　　者：陈鲁琦[1,2,3,4] 陈劲 宋冬平 陈鲁豫[1,2,3,4] 

机构地区：[1]河南省胸科医院呼吸内科 [2]河南省中心血站 [3]郑州市第5人民医院 [4]平顶山市第一人民医院

出　　处：《河南医学研究》1998年第1期45-47,共3页Henan Medical Research

摘　　要：目的：建立银染－ＴＲＡＰ法检测胸水脱落细胞端粒酶活性，探讨银染－ＴＲＡＰ法用于胸水，脱落细胞中端粒酶活性研究的可行性。方法：应用银染－ＴＲＡＰ法检测３０例癌性胸水脱落细胞和３０例结核性胸膜炎胸水脱落细胞端粒酶活性的表达。结果：癌性胸水脱落细胞端粒酶活性为１００％（３０／３０）表达，对照组胸水脱落细胞端粒酶活性为阴性（Ｐ＜０．０１）。结论：癌性胸水脱落细胞中存在端粒酶活性；端粒酶活性与癌性胸水的形成原因有关；银染－ＴＲＡＰ法是一种敏感、可靠且简便、快速和经济的方法，适用于临床实验室进行大规模的样本筛选。Objective: To creat a new technology shiver-staining and TRAP (telomeric repeat amplification protocol) were detected exfoliated cancer cells of chest fluid;to understand the telomerase activity in exfoliated cancer cells of chest fluids and to determine if the sliver-staining technique and TRAP was practicable.Methods: In the paper telomerase activity was examined by a polymerase chain reaction-based assay and sliver-staining technique in exfoliated cells from 30 patients chest fluids of cancer,30 patients tuberculosis pleurisy.Results: Showed that evidence of telomerase was detected in exfoliated cells from 30 patients chest fluid of cancer(100%),whereas it was not found in any tuberculosis pleurisy chest fluid exfoliated cells(P<0.001).Conclusion: The results suggest that telomerase could be a good diagnostic marker for exfoliated cells of cancer chest fluid,the mechanism causing cancer chest fluid may be telomerase activity.Sliver-staining and TRAP is one of most sensitive and simplest measurement for detecting telomerase activity.It is worth using in clinical laboratory because of its readiness and lower cost.

关 键 词：端粒酶 胸水 银染 端粒 脱落细胞 

分 类 号：R561.3[医药卫生—呼吸系统] R730.21[医药卫生—内科学]