反相离子对高效液相色谱法测定人血清中异烟肼浓度  

Determination of Isoniazid by IonPair HPLC in Human Serum

作　　者：石瑞如[1] 余彩莲[1] 谢红歌[1] 尹江伟 

机构地区：[1]河南省胸科医院临床药理室

出　　处：《河南医学研究》1998年第1期70-72,共3页Henan Medical Research

摘　　要：目的：建立一种快速简便的的反相离子对高效液相色谱法测定血清中异烟肼（ＩＮＨ）浓度。方法：用ＳｐｈｅｒｉｓｏｒｂＳｉＯ２（５μ，２５０ｍｍ×４６ｍｍ）色谱柱，以甲醇：２０ｍｍｏｌ／ＬＩＰＲ－Ｂ８＝１５∶８５（Ｖ∶Ｖ）为流动相，紫外检测器检测，波长２５４ｎｍ，烟酰胺（ＮＡ）为内标物。结果：在此色谱条件下，ＩＮＨ与ＮＡ的保留时间分别为７８１７ｍｉｎ和６８７２ｍｉｎ。在０５～２００μｇ／ｍｌ的浓度范围内，线性关系良好（ｒ＝０９９９５）。方法日内差异２１９％，日间差异４５１％，回收率９３３％，最低检测浓度５０ｎｇ／ｍｌ。结论：该法灵敏度高，特异性好，不需提取过程，大大减低了测定的工作量，对日常血药浓度监测和药代动力学工作的开展，具有极大的优越性。Objective:An ionpair HPLC method for the quantitative analysis of isoniazid in human serum was described.Methods:High performance liquid chromatograph Model LC10 A (Shimadzu ,Japan) with a 254 nm wavelength uvdetector and Spherisorb SiO2 column (5u,250×4.6 mm) was used.The mobile phase of methanol and 2.0 mmol/L IPRB8(15:85;V:V,pH 2.5) was pumped at 1.0 ml/min through the column.The temperature of column box was 40℃ and the detector was operated at 0.016 AuFs.Nicotinic amide was used as the internal standard .Results:The retention time for isoniazid and nicotinic amide was 7.817 min ,6.872 min,respectively.Standard curve was linear in the concentration range of 0.5 to 20.0 μg/ml for isoniazid .The recovery rate was 93.3%,withinday RSD was 2.19%,interday RSD was 4.51%,the minimum quantification concentration was 50 ng/ml.Conclusion:This method is rapid and convenient for monitoring the plasma concentration of isoniazid.

关 键 词：异烟肼 高效液相色谱法 血药浓度 

分 类 号：R978.3[医药卫生—药品] R969.1[医药卫生—药学]