hVEGF基因工程生物膜对大鼠全层皮肤缺损创面中Fn和整合素α5表达的研究  

Effects of hVEGF Genetically Engineering Membrane on Expression of Fn and Integrin α5 During Healing Process of Full-thickness Cutaneous Defect of Rats

作　　者：赵雷[1] 王莉[1] 姜叙诚[1] 郑林[1] 张帆[1] 赵涵芳[2] 许伟榕[2] 

机构地区：[1]上海交通大学医学院病理学教研室,上海200025 [2]上海交通大学医学院生物化学与分子生物学教研室,上海200025

出　　处：《实验动物与比较医学》2009年第2期100-104,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的探讨将含人血管内皮细胞生长因子（hVEGF）重组质粒的成纤维细胞种植的基因工程生物膜覆盖于大鼠全层皮肤缺损创面，在创面愈合过程中对细胞外基质中纤维连接蛋白（Fn）及其受体整合素α5表达的影响。方法通过建立hVEGF基因工程生物膜覆盖大鼠全层皮肤缺损创面的动物模型，采用免疫组化方法并结合图像分析，分别观察并测定实验组和对照组大鼠创面愈合3、7、14、29d时创面中Fn和整合素α5表达的平均光密度值，判断其在创面中表达的强弱。结果在术后3、7、14d时，实验组中Fn表达显著高于对照组（P〈0．05）；在术后14、29d时，实验组中整合素α5表达显著高于对照组（P〈0．05）。结论将hVEGF基因工程生物膜覆盖于大鼠全层皮肤缺损创面，在创面愈合的早、中期促进Fn的表达；在创面愈合中、晚期，上调整合素α5的合成与分泌，从而有利于创面愈合。Objective To study the effects of hVEGF on the expression of Fn and integrin A5 during the healing process of rats＇ full-thickness cutaneous defect which covered by genetically membrane planted by fibroblast integrated with hVEGF recombinant. Methods Wounds with full-thickness cutaneous defect were produced on the dorsum of the SD rats. The rats were randomly divided into four groups. For the rats in the experimented group, the wounds were covered with hVEGF genetically engineering membrane. For the three control groups, the wounds were covered with membrane planted by the fibroblast integrated with blank plasmid, blank membrane and Tegaderm membrane respectively. Specimens were obtained at the 3,7,14 and 29 days after injury, immunohistochemistry was employed to detect the changes in Fn and integrina5 expression, image analysis was used to determine changes of these expression level by detecting the mean optical density value of their expression. Results The expression level of Fn in experimental group was significant higher than that in control groups at 3, 7 and 14d after injury, by detecting the mean optical density value of its expression （P〈0.05）. At 14 and 29d after injury, the expression level of Integrinα5 in experimental group was significant higher than that in control groups （P〈0.05）. Conclusion The application of the hVEGF genetically engineering mem- brane on the full-thickness cutaneous defect upregulated the expression of Fn in experimental group, especially in the early and metaphase of wound healing. Integrinα5 expression significantly increased at metaphase and later period of wound healing. These could accelerate the healing process.

关 键 词：人血管内皮细胞生长因子 纤维连接蛋白 整合素Α5 创面愈合 

分 类 号：Q78[生物学—分子生物学] R641[医药卫生—外科学]