脑微血管内皮细胞条件培养液对MIP-1β诱导大鼠小胶质细胞迁移影响的机制  

Cerebral Micro-vascular Endothelial Cells Conditioned Medium Inhibits MIP-1β-Induced Microglial Migration by Down-Regulating the Activation of p38 and JNK

作　　者：王筠[1] 李澎涛[1] 侯金才[1] 华茜[1] 潘彦舒[1] 李卫红[1] 唐卉凌[1] 陈会丛[1] 杜欢[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《现代生物医学进展》2009年第7期1201-1205,共5页Progress in Modern Biomedicine

基　　金：国家"973"计划(2005CB523311);国家自然科学基金(30572284);国家十一五支撑计划(2006BAI08B05-04)

摘　　要：目的:研究经通络方药作用后的脑微血管内皮细胞条件培养液对MIP-1β诱导的大鼠小胶质细胞迁移的影响,以及由MIP-1β在小胶质细胞上的受体-CCR5介导细胞信号转导通路的调控作用。方法:将通络方药作用后的大鼠脑微血管内皮细胞条件培养液加入到由5nMMIP-1β刺激6h后的原代大鼠小胶质细胞中,利用Transwell细胞迁移系统来观察小胶质细胞迁移,用Western blot法检测小胶质细胞CCR5,p-p38,p-JNK表达情况。结果:脑微血管内皮细胞条件培养液能够显著减少小胶质细胞迁移到Transwell下层的细胞数量(P<0.01),降低小胶质细胞上MIP-1β的受体CCR5表达,同时抑制了其下游信号蛋白p38和JNK的磷酸化。结论:通络方药作用后的脑微血管内皮细胞条件培养液可抑制由MIP-1β诱导的大鼠小胶质细胞迁移,其作用发挥可能是通过抑制MIP-1β的受体CCR5表达,降低了其下游通路上p38和JNK蛋白磷酸化程度实现。Objective：We focused our study on the effect of cerebral micro-vascular endothelial cells conditioned medium on microglia migration induced by MIP-1β and its regulation on concerned protein of cell signaling. Method：Add cerebral micro-vascular endothelial cells conditioned medium with Tongluo Jiunao Injection in primary cultured rat microglia stimulated by 5nM MIP-1β for 6h, then observe the microglia migration through Yranswell system, and then detect the protein expression of CCR5, p-p38 and p-JNK on microglia. Result： Cerebral micro-vascular endothelial cells conditioned medium reduced the count of microglia that migrated to the lower side of the filter membrane significantly and inhibited the expression of downstream protein p-p38 and p-JNK. Conclusion：The cerebral micro-vascular endothelial cells conditioned medium with Tongluo Jiunao Injection could significantly inhibit microglia migration induced by MIP-1β , and the mechanism of this effect may be the inhibition to the expression of receptor CCR5 and the phosphorylation of p38 MAPK and SAPK/JNK.

关 键 词：小胶质细胞 趋化因子 迁移 P38 MAPK JNK 

分 类 号：R285.5[医药卫生—中药学] Q95-3[医药卫生—中医学]