重组腺病毒Ad5-ADRbeta2-EGFP转染骨髓间充质干细胞移植治疗心衰大鼠的实验研究  

Experimental Study on Heart Failing Rats Transplanted with BMSCs Transfected by Ad5-ADRbeta2-EGFP

作　　者：宫海滨[1] 王秀力[1] 李春梅[1] 张清林[1] 

机构地区：[1]徐州市心血管病研究所徐州市中心医院,江苏徐州221009

出　　处：《现代生物医学进展》2009年第7期1206-1208,F0002,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30572073);江苏省自然科学基金项目(BK2005428);江苏省"六大人才高峰"(资助类型C);江苏省医学重点人才(RC2007024)。

摘　　要：目的:重组腺病毒Ad5-ADRbeta2-EGFP转染骨髓间充质干细胞(BMSCs)并植入心衰大鼠心肌,观察其心功能的变化。方法:采用部分缩窄腹主动脉法制备大鼠压力超负荷性慢性心力衰竭模型。分离和培养成年大鼠BMSCs,待细胞传代到第6代,用重组腺病毒Ad5-ADRbeta2-EGFP转染BMSCs48h后将BMSCs移植入心衰大鼠心肌内。实验共分3组:心衰组、移植BMSCs组及移植转染Ad5-ADRbeta2-EGFP的BMSCs组。移植BMSCs(1×104)4周后,检测大鼠血流动力学各指标的变化。结果:荧光显微镜下可见转染BMSCs绿色荧光蛋白表达达到80%以上。移植BMSCs组和移植转染Ad5-ADRbeta2-EGFP的BMSCs组的左心室舒张末压力(LVEDP)低于心衰组,左心室内压变化最大速率(±dp/dtmax)和心率均高于心衰组,差异均有统计学意义(P<0.05)。移植转染Ad5-ADRbeta2-EGFP的BMSCs组的左心室内压上升最大速率(+dp/dt)高于移植BMSCs组,差异有统计学意义(P<0.05)。结论:重组腺病毒Ad5-ADRbeta2-EGFP转染BMSCs并移植入心衰大鼠后,能够改善心衰大鼠的心功能。Objective：To study the changes of function of heart failure rat transplanted with bone mesenchymal stem cells（BMSCs）transfected by Ad5-ADRbeta2-EGFP. Methods：Rat model of heart failure （HF） was established by partially banding abdominal aorta. The BMSCs were isolated and cultured in P6 in vitro. Then the BMSCs transfected by Ad5-ADRbeta2-EGFP for 48h were collected and transplanted to heart failing rat in 1 × 10^4. The experiment was divided into three groups： HF group, HF＋ BMSCs group and HF＋ADRbeta2-BMSCs group. After four weeks, the changes of haemodynamics were measured in heart failing rats. Results：After BMSCs were transfected by Ad5-ADRbeta2-EGFP for 48h, the expression of green fluorescence protein was more than 80%. Compared with HF group, the LVEDP of heart was decreased significantly in HF＋BMSCs group and HF＋ADRbeta2-BMSCs group （P〈0.05）, and the parameters of ± dp/dt and heart rate （HR）were significantly increased （P 〈 0.05）. Furthermore, the parameter of ＋dp/dt of HF＋ADRbeta2-BMSCs group was higher than that of HF＋ BMSCs group （P 〈0.05）. Conclusions：The results suggested that the cardiac function could be improved significantly in heart failing rats transplanted with BMSCs transfected by Ad5-ADRbeta2-EGFP.

关 键 词：心力衰竭 骨髓间充质干细胞 基因转染 beta2肾上腺素能受体 心功能 大鼠 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R541.6[医药卫生—基础医学]