新型PEI衍生物对基因沉默作用的研究  

Effect of a Novel PEI Derivative on the Gene Silencing

作　　者：陈默颖[1] 何沐[1] 陆平[1] 杜子秀[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《现代生物医学进展》2009年第7期1260-1261,1264,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30873180)

摘　　要：目的:构建出一种新型的高效的非病毒基因输送材料。方法:利用1,4-丁二醇二氯甲酸酯连接小分子PEI,合成新型的PEI衍生物(dPEI),通过体外COS-7和HSC细胞实验检测其对质粒和siRNA的输送情况。结果;COS-7细胞实验显示,dPEI具有高效输送质粒的能力,其转染效率是对照组PEI25kDa的10倍以上;同时,HSC细胞实验进一步证实dPEI包裹siRNA转染具有一定的基因沉默作用。结论:本研究合成的新型PEI衍生物既可以用于质粒基因的转染,也可用于siRNA的转染,是一种新型的高效的非毒基因输送材料。Objective：To synthesize a novel and efficient non-viral gene delivery vector. Methods：1, 4-bisbutanediol chloroformate was used to link small molecular PEI to synthesize a novel PEI derivative （dPEI） and the gene delivery efficiency was further determined by cells experiments. Results：DPEI had high transfection efficiency to the COS-7 cells, almost 10 times as that of PEI 25kDa, with certain activity of gene silencing by delivering siRNA to HSC. Conclusions：Here a novel PEI derivative was reported as a non-viral gene delivery carriers and it can be used in plasmid and siRNA delivery.

关 键 词：SIRNA沉默 PEI衍生物 转染 

分 类 号：R287[医药卫生—中药学]