桂枝茯苓微丸的质量标准研究  

Study of Quality Standard for Guizhi Fuling Micropill

作　　者：闫雪生[1] 李岩[1] 郑亭亭[2] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东大学,山东济南250100

出　　处：《中华中医药学刊》2008年第12期2555-2556,共2页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的：建立桂枝茯苓微丸的质量标准。方法：采用薄层色谱法对丹皮、赤芍、茯苓进行了定性鉴别,采用高效液相色谱法测定了微丸中芍药苷的含量,采用Lichrospher C18柱（5μm,200mm×4.6mm）;检测波长230nm;流动相：乙腈-水-磷酸（15：85：0.1）;流速：1.0mL/min;柱温：室温。结果：此法线性范围1.506-2.550μg,Y=64.3537924X＋145.99898,r=0.9996;芍药苷的平均回收率为97.04%,RSD为2.03%。结论：该方法稳定、可靠,可作为该制剂的质量控制方法。Objective： To establilsh the quality standard for Guizhi fuling micropill.Methods：TLC was performed to identify Cortex moutan,Paeoniae radix and Poria.HPLC was used to determine Peoniflorin.Lichrospher C18 column（5μm,200mm×4.6mm） was used.The detecting wave length was at 230 nm.The mobile phase consisted of acetonitrile-water-phosphoric acid（15∶85∶0.1）.The flow rate was 1.0 mL/min and column temperature was at room temperature.Results：A good linearity was shown in the concentration range of 1.506～2.550μg,Y=64.3537924X＋145.99898,r=0.9996.The average recovery of Peoniflorin was 97.04%,RSD was 2.03%.Conclusion：The method is convenient,reliable and accurate for the quality control of Guizhi fuling micropill

关 键 词：桂枝茯苓微丸 质量标准 薄层色谱 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]