血清中抗水痘病毒IgG抗体的检测方法  

Determination Methods for IgG against Varicella-Zoster Virus in Sera

作　　者：宋颖丽[1,2] 姜典财[2] 李长贵[2] 

机构地区：[1]昆明理工大学生物工程技术研究中心,昆明650224 [2]中国药品生物制品检定所,北京100050

出　　处：《中国生物制品学杂志》2009年第4期415-416,424,共3页Chinese Journal of Biologicals

摘　　要：水痘是由水痘-带状疱疹病毒引起的一种疾病,主要见于儿童,在人群中具有极高的感染率。IgG是机体感染水痘后产生的两种主要中和抗体之一,检测血清中水痘IgG抗体在水痘血清流行病学研究、水痘疫苗的免疫效果评价、临床诊断等方面具有重要意义。本文主要对检测血清中水痘IgG抗体敏感性和特异性较高的膜抗原荧光抗体法、酶联免疫吸附法、间接免疫荧光抗体法、免疫吸附血凝试验法、补体扩大中和试验法和放射免疫法作简要介绍。Varicella is a disease mainly epidemic in children. With a high infection rate in population,the disease was caused by varicella-zoster virus（VZV）. IgG is one of the major neutralizing antibodies induced after infection with VZV. The determination of IgG against VZV in sera is of important significances in seroepidemiological study and clinical diagnosis of varicella as well as evaluation of immune effect of varicella vaccine. The sensitive and specific methods for determination of IgG against VZV in sera,such as FAMA,ELISA,IFA,IAHA,CENTLA and RIA,are reviewed in this paper.

关 键 词：水痘-带状疱疹病毒 IGG 检测方法 

分 类 号：R392[医药卫生—免疫学] R44[医药卫生—基础医学]