电针对股骨骨折大鼠骨痂内神经肽表达水平的影响  

Electroacupuncture and the expression of neuropeptides in the callus formed after femoral fracture

作　　者：王新[1] 周路纲[1] 刘洪智[1] 王磊[1] 孙煜杰[1] 苏昊[1] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院创伤骨科,烟台264000

出　　处：《中华物理医学与康复杂志》2009年第4期245-248,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的研究电针刺激穴位对股骨骨折大鼠骨痂内神经肽表达水平的影响。方法将40只雄性Wistar大鼠随机分为骨折组和电针组，2组大鼠均制成股骨骨折模型，其中电针组大鼠于骨折模型建立后给予电针刺激。2组大鼠分别于术后第4，7，14及28天时各取5只处死，并采集骨折部位组织制成切片。采用免疫组织化学染色法检查各组大鼠术后不同时间点骨痂内降钙素基因相关肽（CGRP）及P物质（SP）表达情况。结果电针组大鼠在术后骨折愈合不同阶段，其骨痂内各种细胞胞浆中均有CGRP、SP阳性颗粒表达，且出现时间较骨折组早，维持时间较骨折组长；图像分析系统显示电针组骨痂内CGRP、SP光密度值明显高于骨折组，组间差异具有统计学意义（P〈0．05）。结论电针刺激穴位能显著提高骨折大鼠骨痂内CGRP、SP等神经肽表达，推测电针可通过影响神经肽水平参与并调节骨折愈合过程。Objective To investigate the influence of electroacupuncture on the expression of neuropeptides in fibrocartilage callus tissue after femoral fracture. Methods Forty male Wistar rats were randomly divided into a fracture group and an electroacupuncture group. Femoral fracture models were established in both groups, and the rats in the electroacupuncture group were given electroacupuncture. Rats were sacrificed on the 4th, 7th, 14th and 28th days after surgery. The calluses were stained immunohistochemically to detect the expression of calcitonin generelated peptide （CGRP） and substance P （SP）. Results CGRP and SP levels increased rapidly after electroacupuncture, with neuropeptides expressed strongly in callus cell tissue. The optical density （OD） in the electroacupuncture group was significantly higher than that in the fracture group （P≤0.05）. Conclusion Electroacupuncture can significantly increase the expression of neuropeptides in rats after femoral fracture. Electroacupuncture can promote fracture healing through regulating neuropeptides.

关 键 词：神经肽 电针 骨痂 股骨骨折 

分 类 号：R245[医药卫生—针灸推拿学]