表没食子儿茶素没食子酸酯对结肠癌HT-29细胞MMP-9表达的影响  

Effect of Epigallocatechin-3-gallate(EGCG) on MMP-9 Expression in Human Colon Cancer Cell Line HT-29 in Vitro

作　　者：周萍[1] 陈卫昌[2] 张淳[1] 俞宪民[1] 

机构地区：[1]无锡市第三人民医院,214000 [2]苏州大学附属第一医院

出　　处：《天津医药》2009年第4期248-250,共3页Tianjin Medical Journal

基　　金：江苏省135重点医学人才基金资助课题(项目编号:37RC2007076)

摘　　要：目的:探讨表没食子儿茶素没食子酸酯(EGCG)对人结肠癌细胞株HT-29基质金属蛋白酶-9(MMP-9)表达的影响。方法:应用RT-PCR法和Western blot法检测EGCG干预前后HT-29结肠癌细胞中MMP-9mRNA及MMP-9蛋白表达。结果:EGCG25、50、100mg/L作用于结肠癌细胞株HT-2948h后,与对照组比较,MMP-9mRNA及MMP-9蛋白的表达量随着EGCG浓度的增加而下降,且各浓度组间差异也有统计学意义(P<0.05)。结论:EGCG可显著抑制HT-29细胞中MMP-9mRNA及MMP-9蛋白的表达,呈剂量依赖性,可能是其抗癌机制之一。Objective ： To study the effect of Epigallocatechin-3-gallate（EGCG） on Matrix metalloproteinase 9（MMP-9） in human colon cancer cell line HT-29 in vitro. Methods： In vitro, the MMP-9 mRNA and protein expression in human colon cancer cell line HT-29 were analyzed by RT-PCR and Western blot. Results： In vitro, human colon cancer cell line HT-29 was treated by various concentrations of EGCG （25, 50, 100 mg/L）. After 48 hours, the expression of MMP-9 mRNA and protein in human colon cancer cell line HT-29 were gradually decreased with the increasing dose of EGCG （P 〈 0.05）. Conclusion： EGCG can inhibit the expression of MMP-9 in HT-29 cell in vitro and in dose dependent manner, which may be one of the mechanisms of anti-tumor effect of EGCG.

关 键 词：儿茶素没食子酸 结肠肿瘤 癌 肿瘤细胞 培养的 明胶酶B 

分 类 号：R282.71[医药卫生—中药学] R739.65[医药卫生—中医学]