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机构地区:[1]泸州医学院病理教研室,四川泸州646000 [2]泸州医学院附属医院肾病内科,四川泸州646000 [3]泸州医学院医学实验中心,四川泸州646000
出 处:《中国医师杂志》2009年第4期450-452,共3页Journal of Chinese Physician
基 金:四川省科技厅应用基础研究资助项目(05JY029-103-2)
摘 要:目的探讨血管紧张素-(1-7)[Ang-(1-7)]对转化生长因子-β1(TGF-β1)诱导大鼠系膜细胞(GMC)增殖和细胞外基质分泌的影响。方法选择对数生长期GMC,分为对照组、Ang-(1-7)组、TGF-β1组、TGF-β1+Ang-(1-7)组,采用WST-1检测系膜细胞增殖,RT-PCR法和放射免疫法检测细胞外基质层粘蛋白(LN)、Ⅳ型胶原(COLIV)的表达。结果与对照组相比,Ang-(1-7)组细胞数目和细胞外基质减少(P〈0.05);与TGF-β1组相比,Ang-(1-7)可抑制TGF-β1诱导的系膜细胞增殖、使细胞内LNmR-NA、COLⅣ mRNA表达量明显下调、同时导致上清液中细胞外基质LN、COLⅣ含量显著减少(P〈0.05)。结论Ang-(1-7)能抑制基础和TGF-β1诱导的GMC细胞增殖及细胞外基质的分泌,可能在抑制肾脏纤维化过程中发挥作用。Objective To explore the effect of angiotensin-( 1-7 ) [ Ang-( 1-7 ) ] on proliferation and secretion in cultured rat's glomerular mesangial cells(GMC) induced by transforming growth factor-β1 (TGF-β1). Methods GMC in logarithmic growth phase were incubated, and then were divided into 4 groups: control, Ang-( 1-7), TGF-β1,and TGF-β1 + Ang-(1-7) group. Cell numbers of rat's GMC were detected by WST-1. Laminin(LN) and collagenⅣ (ColⅣ) mRNA expressions in GMC were analyzed by RT-PCR. The secretion of LN and Col lV in culture medium of rat's GMC was measured by radioimmunoassay. Results Ang-(1-7) significantly inhibited basal and TGF- β1-induced proliferation of cultured glomerular mesangial cells. Ang-(1-7) significantly down-regulated LN and ColⅣ mRNA expressions in glomerular mesangial cells and also inhibited the secretion of LN and Col IV induced by TGF-β1( P 〈 0. 05 ). Conclusions Ang-( 1-7 ) can inhibit basal and TGF-β1-induced proliferation, and inhibit ECM secretion of cultured rat's glomerular mesangial ceils.
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