海南长春花黄化病植原体的16S rDNA序列分析研究  

Sequence analysis of 16S ribosomal DNA of phytoplasma associated with periwinkle yellows disease in Hainan

作　　者：车海彦[1,2] 罗大全[2] 符瑞益[2] 叶莎冰[2] 吴云锋[1] 

机构地区：[1]西北农林科技大学植物保护学院陕西省农业分子生物学重点实验室,杨凌712100 [2]中国热带农业科学院环境与植物保护研究所,儋州571737

出　　处：《植物病理学报》2009年第2期212-216,共5页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30660103)

摘　　要：Periwinkle(Catharanthus roseus) yellows is a common disease in Hainan. Periwinkle′s leaf tissue with symptoms was assayed for phytoplasma infection by using PCR assay employing phytoplasma universal 16S rRNA gene primers (Rl6mF2/Rl6mR1). A PCR product (about 1.4 kb) was amplified from periwinkle showed yellows. Nucleotide sequencing and phylogenetic tree analysis showed that the amplified 16S rDNA contained 1 432 nucleotides, the most homology was 98.1% with the members of elm yellows group (16S r Ⅴ) and clustered in the same clade, while it was under 96.1% with other phytoplasma groups. Our results suggested that the phytoplasma sample belonged to 16S rⅤgroup and was tentatively named as Hainan periwinkle yellows phytoplasma (PY-Hn). This is the first report of existence of 16S r Ⅴ group phytoplasma in naturally infected periwinkle.Periwinkle（Catharanthus roseus） yellows is a common disease in Hainan. Periwinkle′s leaf tissue with symptoms was assayed for phytoplasma infection by using PCR assay employing phytoplasma universal 16S rRNA gene primers （Rl6mF2/Rl6mR1）. A PCR product （about 1.4 kb） was amplified from periwinkle showed yellows. Nucleotide sequencing and phylogenetic tree analysis showed that the amplified 16S rDNA contained 1 432 nucleotides, the most homology was 98.1% with the members of elm yellows group （16S r Ⅴ） and clustered in the same clade, while it was under 96.1% with other phytoplasma groups. Our results suggested that the phytoplasma sample belonged to 16S rⅤgroup and was tentatively named as Hainan periwinkle yellows phytoplasma （PY-Hn）. This is the first report of existence of 16S r Ⅴ group phytoplasma in naturally infected periwinkle.

关 键 词：RDNA序列分析 植原体 长春花 黄化病 多年生草本植物 16S ASTER 海南 

分 类 号：S436.65[农业科学—农业昆虫与害虫防治] Q939.1[农业科学—植物保护]