瘦素、纤连蛋白、基质金属蛋白酶-9对人绒毛细胞滋养细胞侵袭性的影响  

Effects of leptin,fibronectin and MMP-9 on the invasion of human cytotrophoblast

作　　者：杨蕾[1] 杨玲竹[1] 

机构地区：[1]郑州大学第一附属医院,河南郑州450052

出　　处：《山东医药》2009年第15期1-2,共2页Shandong Medical Journal

基　　金：河南省教育厅自然科学研究计划资助项目(2008A320051)

摘　　要：目的探讨瘦素、纤连蛋白（FN）、基质金属蛋白酶-9（MMP-9）对人绒毛细胞滋养细胞侵袭性的影响。方法取孕6-10周人工流产妊娠绒毛，分离、培养细胞滋养细胞（CTB）并经光镜、免疫细胞化学鉴定；应用体外培养侵袭模型检测瘦素、FN、MMP-9及瘦素＋FN对CTB侵袭能力的影响。结果瘦素、FN、MMP-9及瘦素＋FN作用后，浸润穿过基底膜的CTB数均显著多于对照组（P均〈0．01），尤其是瘦素＋FN。结论瘦素、FN、MMP-9能增强CTB的侵袭能力；瘦素与FN有协同作用。Objective To investigate the effects of leptin, fibronectin （FN） and MMP-9 and on the invasion of human cytotrophoblast（CTB）. Methods CTB were isolated from chorionic villi at 6 - 10weeks pregnancy,valuated by light microscope and immunocytochemistry. The effects of leptin ,fibronectin and MMP-9 on the invasion of CTB were detected by using invasion model. Results The number of penetrated-membrane-cells in leptin, FN, MMP-9,1eptin ＋ FN groups were signifi- cantly higher than that in the control group（ P 〈 0.01 ）. In particular which in the leptin ＋ FN group. Conclusions Leptin, FN, MMP-9 can increase the invasion ability of CTB. Leptin and FN have synergy.

关 键 词：瘦素 纤连蛋白 基质金属蛋白酶-9 细胞滋养细胞 侵袭 

分 类 号：R714.2[医药卫生—妇产科学]