壳聚糖修饰的神经毒素纳米粒的制备及体外释放研究  

Preparation and in vitro release of neurotoxin-loaded PLA nanoparticles modified with chitosan

作　　者：柴国宝[1] 魏颖慧[1] 冯健[1] 程巧鸳[1] 李范珠[1] 

机构地区：[1]浙江中医药大学药学院,杭州310053

出　　处：《中国药科大学学报》2009年第2期116-119,共4页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.30371781;No.30772793);浙江省卫生高层次创新人才培养工程~~

摘　　要：目的:制备粒径小、形态均匀、包封率较高、带稳定正电荷的神经毒素纳米粒并研究其体外释放行为。方法:选用聚乳酸为载体,壳聚糖为修饰物,采用复乳法制备神经毒素纳米粒,在单因素试验的基础上结合正交试验优化纳米粒的制备工艺,并对其体外释药特性进行研究。结果:采用优化方法制备的纳米粒粒径较小(140.5nm),形态规则,大小均匀,包封率高(83.5%),Zeta电位为+30.5mV;体外释药行为符合Weibull方程。结论:建立的制备工艺可行,所得纳米粒包封率高,大小均匀,体外释药具有明显的缓释特征。Aim： To prepare the neurotoxin chitosan-modified PLA nanoparticles with small diameter, good mor- phology, high drug-loading encapsulation efficiency and positive Zeta potential. Methods： The nanoparticles were prepared with PLA and ehitosan by the double-emulsion method, and the orthogonal design was used to optimize the processing parameters, and a preliminary study on the drug release in vitro was made. Results： It was found that the prepared nanoparticles had small particle （ 140. 5 nm） and good morphology with high encapsulation effi- ciency （83.5%） and positive Zeta potential of ＋ 30. 5 mV. In vitro release profile of the nanoparticles was best fitted to Weibull equation. Conclusion： The established method is practicable in the preparation of neurotoxin chitosan-modified PLA nanoparticles with high encapsulation efficiency, narrow range of the particle size distribution, and notable sustained-release in vitro.

关 键 词：神经毒素 纳米粒 壳聚糖 复乳法 体外释放 

分 类 号：R944[医药卫生—药剂学]