蜂胶的HPLC指纹图谱研究  

HPLC fingerprint of propolis

作　　者：沙娜[1,2] 黄慧莲[1,2] 张金强[2] 李萍[1] 果德安[2] 

机构地区：[1]中国药科大学中药学院,南京210009 [2]中国科学院上海药物研究所上海中药现代化研究中心,上海201203

出　　处：《中国药科大学学报》2009年第2期144-146,共3页Journal of China Pharmaceutical University

摘　　要：目的:应用反相高效液相色谱法建立蜂胶药材指纹图谱,对蜂胶进行质量控制。方法:采用Zorbax Extend-C18(250mm×4.6mm,5μm)为分析色谱柱,以甲醇-0.3%醋酸水溶液为流动相测定了18批不同来源蜂胶的HPLC指纹图谱,进行了相似度评价。结果:该方法可使蜂胶中各成分得到较好的分离,根据检测结果确定了15个共有指纹峰,并指认了其中的14个成分。结论:该指纹图谱检测方法简便、重复性好,可用于蜂胶原料的质量控制。Aim： To study the fingerprints of propolis by RP-HPLC and conduct the quality control of propolis. Methods： 18 batches of propolis from different sources were analyzed on an HPLC column Zorbax Ex- tend-Cl8 （250mm×4.6mm,5μm） using a mobile phase consisting of methanol and 0. 3 % aqueous acetic acid, and the obtained data were analyzed using the software Computer Aided Similarity Evaluation. Results： Sound separation of the chemical constituents of propolis were achieved, among which 15 fingerprint peaks in common were confirmed and 14 peaks were designated. Conclusion： The method is repeatable and reliable, and could be applied in the quality control of propolis.

关 键 词：蜂胶 高效液相色谱 指纹图谱 质量控制 

分 类 号：R284[医药卫生—中药学]