肠缺血再灌注损伤后RSpo1和β-catenin在肠上皮的表达及作用  

The expression and efffects of RSpo1 and β-catenin in intestinal epithelium with intestinal ischemia-reperfusion injury in mouse

作　　者：殷刚[1] 吴承堂[1] 雷尚通[1] 王运星[1] 

机构地区：[1]南方医科大学南方医院普通外科,广州广东510515

出　　处：《中国普通外科杂志》2009年第4期367-370,共4页China Journal of General Surgery

基　　金：广东省自然科学基金资助项目(032901)

摘　　要：目的探讨肠缺血再灌注损伤(IIRI)后肠上皮R-Spondin1(RSpo1)mRAN和β-链接素(β-catenin)mRAN的变化及其作用机制。方法健康雄性昆明小鼠50只,分为对照组(10只)和实验组(40只)。对照组仅作剖腹手术。实验组小鼠麻醉后夹闭肠系膜动脉20min后松夹,然后再分为4组,即再灌注6h(A组),12h(B组),24h(C组)和48h(D组)组。采用RT-PCR方法检测小肠组织RSpo1和β-cateninmRNA的表达。结果IIRI后6,12,24,48h小肠绒毛高度逐渐递增,前3个时点显著低于对照组(P<0.01),48h也显著低于对照组(P<0.05)。β-catenin表达变化和小肠绒毛高度变化趋势一样,但其表达均显著低于对照组(P<0.01)。RSpo1表达在IIRI后6h显著降低于对照组(P<0.05),12h表达逐渐增加,24h达到高峰并显著高于对照组(P<0.01);随后其表达迅速下降,48h显著低于对照组(P<0.05)。结论缺血再灌注损伤能抑制β-catenin和早期RSpo1表达,诱导中期RSpo1表达。两者均与肠道上皮干细胞增殖和分化有关,参与肠黏膜受损后的修复过程。Objective To explore the expression and effects of RSp1 and β-catenin in the intestinal epithelium with intestinal ischemia-reperfusion injury （ IIRI ） in of mouse. Methods Fifty healthy male kunming mice were randomly divided into control group （n = 10 ）and experimental group （n = 40 ）. All mice in control group were only subjected to laparotomy, while the other mice underwent 20 minutes of intestinal mesenteric artery occlusion followed by 6 hours （ group A ） , 12 hours （ group B ） , 24 hours （ group C ） and 48 hours（ group D） of reperfusion. RT-PCR was used to detect RSpo1 and β-catenin in small intestine in intestinal ischemia-repeffusion groups and in control group. Results The villous heights of intestinal in experimental groups were significantly lower than that in control group （ P 〈 0.05 - 0.01 ） . The varying trend of β-Catenin expression in experimental groups was the same as villous height, its expression was significantly weaker than that in control group （ P 〈 0. 01 ） , and increased gradually along with the reperfusion time. Compared with control group, RSpo1 expression decreased rapidly at 6h （ P 〈 0. 05 ） , then increased gradually at 12 h, reached the peak level at 24h （ P 〈 0. 01 ） followed by rapid decrease and remained significantly lower than that in control group at 48h （ P 〈 0.05 ）. Conclusions Intestinal ischemia-reperfusion injury may inhibit β-catenin and RSpo 1 expression in early stage, but it increase in middle stage. Both of them may be related with the proliferation and differentiation of intestinal stem cells and role an important part in the process of repair after mucosal damage.

关 键 词：肠缺血再灌注损伤 R-Spondin1 Β-CATENIN 肠上皮干细胞 

分 类 号：R656.7[医药卫生—外科学]