组织仿体液中近红外荧光分子探针的3-D成像  

3-D Image of Near-infrared Fluorescent Probe in Tissue-like Solution.

作　　者：胡刚[1] 姚俊杰[1] 宋小磊[1] 白净[1] 

机构地区：[1]清华大学生物医学工程系,北京100084

出　　处：《航天医学与医学工程》2009年第2期135-138,共4页Space Medicine & Medical Engineering

基　　金：863项目(2006AA020803);973项目(2006CB705700)

摘　　要：目的研制荧光分子断层成像实验系统,获取组织仿体内近红外荧光探针的3-D图像。方法系统采用低温制冷CCD相机作为高灵敏弱光信号探测器,通过旋转目标物,得到各个角度的激发荧光场。组织内近红外光子传输遵循漫射近似理论,图像重建采用基于有限元的快速算法。结果荧光检测系统可以获取全周高密度原始数据,重建图像可再现漫射介质内的荧光目标物的位置与3-D形状。结论仿体液中荧光目标物重建结果良好,可作为在体动物光学断层成像平台。Objective To develop the fluorescence molecular imaging system and obtain the 3 D images of nearinfrared fluorescent probe in tissue-like solution. Methods Weak optical signals were acquired with hypothermal cooled CCD camera. The system captured the fluorescent images of different projections at rotating the tissue phantom. The photon propagation in tissue followed the diffusion approximation theory. The 3D image of probe was calculated with a fast reconstruction algorithm based on finite element approach. Results The imaging system could obtain the full-angle dense raw dataset. The reconstruction algorithm had capacity to recover the position and contour of the fluorescent target included in diffusive medium. Conclusion The results from phantom experiments are promising and show the potentials to act as a platform for in vivo fluorescence tomography.

关 键 词：荧光分子断层成像 光学成像 3-D重建 组织仿体液 

分 类 号：R318.51[医药卫生—生物医学工程]