蛋白质组学技术研究进展  

The Progress of the Proteomic Technology.

作　　者：刘军莲[1] 李勇枝[1] 高建义[1] 盖玉清[1] 王静[1] 薛春美[1] 辛冰牧[1] 

机构地区：[1]中国航天员科研训练中心,北京100193

出　　处：《航天医学与医学工程》2009年第2期151-156,共6页Space Medicine & Medical Engineering

基　　金：国际科技合作项目(2006DFA32250);国家自然基金面上项目(30772694)

摘　　要：随着现代科学技术的飞速发展,现代生物学技术已经发展到后基因组时代,包括人类在内的几十种生物体基因组测序已经完成,生命科学研究的重点从结构基因组研究转移到后基因组时代——功能基因组的研究,蛋白质组学研究是功能基因组一个重要的组成部分。因此,与之相适应,蛋白质组学研究技术也是日新月异,层出不穷,除了最经典的二维凝胶电泳技术,多维液相色谱技术,同位素标记亲和标签技术相继发展起来,蛋白质芯片技术及噬菌体展示技术也得到了较为广泛的应用。本文针对蛋白质组学研究上述关键技术进行了综述。With the rapid development of modern science and technology, the post-genomics period has come with the complement of the sequence of body genome including several tens of human genome, the emphasis of life science transfer from instruction genome to the post-genomics period, functional genome. Proteomics is the most important part of it. The technology of proteomics is advanced day by day. Except the classical two dimensional gel electrophoresis, the technology of multi-dimensional liquid chromatography and the technology of isotope coded-affinity tags have been successively developed, as well as protein chip and phage display have been applied extensively. This article simply summarizes the key technologies of proteomics.

关 键 词：蛋白质组学 二维凝胶电泳 多维液像色谱 同位素标记亲和标签技术 蛋白质芯片 噬菌体展示技术 

分 类 号：Q789[生物学—分子生物学]