野菊花高效液相色谱指纹图谱及质量评价方法的建立  

Quality Evaluation of Flos Chrysanthemi Indici by HPLC Fingerprint and Component Analysis

作　　者：刘婷娜[1] 朱恩圆[1,2] 侴桂新[1,2] 王峥涛 

机构地区：[1]上海中医药大学中药研究所中药标准化教育部重点实验室,上海201203 [2]上海中药标准化研究中心,上海201203

出　　处：《时珍国医国药》2009年第4期823-825,共3页Lishizhen Medicine and Materia Medica Research

基　　金：上海市科委资助项目(No.05DZ19742)

摘　　要：目的建立野菊花的高效液相色谱指纹图谱及质量评价方法,研究不同采集地和不同种野菊花药材的质量。方法利用RP-HPLC方法,分析了12批市售和8批采自不同产地的野菊花药材。流动相:A相为乙腈,B相为0.1%醋酸水溶液,梯度洗脱,流速:1.0 ml.min-1;检测波长:326 nm。结果在45 min内建立了野菊花HPLC指纹图谱,确定了野菊花药材中的22个共有峰,归属了其中的黄酮类化合物,并对其中黄酮类成分进行主成分分析。结论野菊花药材的反相高效液相色谱指纹图谱,重复性好,在野菊花分类学上有一定的指导意义,用于野菊花的质量评价可行。Objective To evaluate the quality of Flos Chrysanthemi Indici by HPLC fingerprint and the component analysis. Methods RP - HPLC was used for the determination of fingerprint. The separation was achieved on a C18 column （250 mm.4.6 mm, 5 μm）. The mobile phase was a mixture of acetonitrile（A） and 0.01% Acetic acid（B） in gradient elution, the flowrate was 1.0 ml . min^- 1, and the detection wavelength was 326 nm. Results The HPLC fingerprint of Flos Chrysanthemi Indici was set up within 45 minutes and 22 characteristic peaks were shown to compare the flavonoids in different origins and different species in Flos Chrysanthemi Indici. Conclusion The RP - HPLC fingerprint method is repeatable, feasible in the quality control of Flos Chrysanthemi Indici and can be used to identify the different species of Flos Chrysanthemi Indici.

关 键 词：野菊花 高效液相色谱法 指纹图谱 质量评价 

分 类 号：R284.1[医药卫生—中药学]