不同浓度粒巨噬细胞集落刺激因子在未成熟树突状细胞培养中的效果研究  

Generation of immature dendritic cells with different dose of granulocyte-macrophage colony-stimulating factor

作　　者：李闻颖[1] 邓锋[1] 王豫蓉[1] 陈允嘉[1] 胡辉[1] 

机构地区：[1]重庆医科大学附属口腔医院正畸科,重庆400015

出　　处：《重庆医科大学学报》2009年第4期424-427,共4页Journal of Chongqing Medical University

摘　　要：目的:探讨不同浓度细胞因子在培养小鼠骨髓来源未成熟树突状细胞(Dendriticcells,DC)中的作用,寻找培养所需最佳细胞因子浓度。方法:分别用低、中、高剂量粒细胞-巨噬细胞集落刺激因子(Granulocyte-macrophagecolony-stimulatingfactor,GM-CSF)联合或不联合白细胞介素4(Interlenkin-4,IL-4)培养小鼠骨髓源性未成熟DC,对其进行形态学观察和细胞表型检测。结果:培养第7d,低剂量组DC数量最少、突起细小,CD80、CD86及主要组织相容(Major histo compatibilitycomplex,MHCⅡ)类分子表达低,如联合IL-4培养可提高DC数量;中等剂量组与高剂量组DC较低剂量组为多,CD80、CD86及MHCⅡ类分子表达亦较前者提高,但二组无明显差异,有无联合IL-4培养的差异亦不显著。结论:GM-CSF低剂量时,可诱导出纯度较高的未成熟DC,而GM-CSF中等剂量和高剂量时,无论是否联合诱导IL-4培养,诱导未成熟DC的产量及纯度无明显差异。Objective：To demonstrate the effect of cytokine with different concentration in mouse bone marrow-derived immature Des and find the optimal cytokine dose for DC culture. Methods： mouse bone marrow-derived immature DCs were cultured in the presence of low, moderate and high dose of GM-CSF cytokine with or without IL-4. The morphology of cells were observed and the phenotype were detected. Results：On day 7, DCs in low dose group had the least quantity, small cell process and low expression of CDS0, CD86 and MHC class II. The quantity of DCs increased if cultured with IL-4 ; DCs in moderate dose group and high dose group were more than that in low dose group and the expression of CD80,CD86 and MHC class II were higher,but it is no difference between moderate dose group and high dose group, however cultured with or without IL-4. Conclusion ：Higher purity of immature DCs could be get when cultured with low dose of GM-CSF. It was no difference of yield and purity of immature DCs when cultured with moderate or high dose of GM-CSF whether or not combine IL-4.

关 键 词：树突状细胞 粒细胞-巨噬细胞集落刺激因子 白细胞介素-4 

分 类 号：R392[医药卫生—免疫学] Q813[医药卫生—基础医学]