高效液相色谱法测定阿折地平的含量  

DETERMINATION OF CONTENT OF AZELNIDIPINE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：薛娜[1,2] 张恺[1] 杜玉民[1] 李林[1] 谷建敏[1] 

机构地区：[1]河北医科大学药学院药物化学教研室,河北石家庄050017 [2]河北化工医药职业技术学院制药工程系药学教研室,河北石家庄050026

出　　处：《河北医科大学学报》2009年第4期373-374,共2页Journal of Hebei Medical University

摘　　要：目的建立高效液相色谱法测定阿折地平的含量。方法采用 Diamosil^(TM)钻石 C_(18)柱(4.6 mm×250mm,5μm)(迪马公司),流动相为乙腈-水(70:30),检测波长为254 nm;流速为1.0 mL/min,进样20μL。结果阿折地平在0.01～0.40 mg/mL 范围内与峰面积呈良好的线性关系(r=0.999 9),阿折地平的含量为99.3%。结论采用高效液相色谱法测定阿折地平的含量,方法简单、可靠,重现性良好,可用于阿折地平的质量控制。Objective To establish a method to determination of content of Azelnidipine by high performance liquid chromatography（HPLC）. Methods The HPLC system was consisted of a DiamosilTM C18 column （4.6 mm×250 mm,5 um）. Acetonitrile and water （70 ： 30） was used as mobile phase,and the detection wavelength was set at 254 nm. The flow rate was 1.0 mL/min. Results The linear range of Azelnidipine was 0.01-0.40 mg/mL（ r =0. 999 9）. The content of Azelnidipine was 99.3%. Conclusion The method is simple, accurate. It can be used for determination of Azelnidipine content.

关 键 词：阿折地平 色谱法 高压液相 质量控制 

分 类 号：R972.4[医药卫生—药品]