日本毛连菜中多糖的含量测定  

Determination of polysaccharide in Picris japoncia Thunb

作　　者：张志勇[1] 席啸虎[1] 康曌[1] 高建平[1] 

机构地区：[1]山西医科大学药学院中药学教研室,太原030001

出　　处：《山西医科大学学报》2009年第4期329-331,共3页Journal of Shanxi Medical University

基　　金：山西医科大学学生创新基金资助项目(2006045)

摘　　要：目的建立药用植物日本毛连菜中多糖的含量测定方法。方法采用水提醇沉法从日本毛连菜中提取得到毛连菜粗多糖,脱色,用苯酚-硫酸法测定日本毛连菜中多糖含量。结果在3.03-15.10μg/ml范围内具有良好的线性关系,本法平均回收率为(97.55±2.26)%,RSD为2.3%。测定日本毛连菜提取物中总多糖含量以葡萄糖计平均为(5.80±0.06)%。结论该方法简便、准确、可靠,可以用于日本毛连菜中多糖的含量测定。Objective To establish a method for determining the content of polysaccharide in Picris japoncia Thunb. Methods Poly- saccharide was extracted with water, purified with alcohol precipitation and decolored with H2O2. Then the content of polysaccharide was determined by phenols-sulfuric acid method. Results The linear range for polysaccharide was 3.03 - 15.10 μg/ml. The average re- covery was （97.55 ± 2.26 ） %. RSD was 2.3 %（ n = 6 ）. The content of polysaccharide in Picris japoncia Thunb was （ 5.80 ± 0.06 ） % （glucose）. Conclusion The proposed method is convenient, economic, accurate and reproducible, and can be used to determine the polysaccharide in Pieris japoncia Thunb.

关 键 词：日本毛连菜 多糖 含量测定 

分 类 号：R927.2[医药卫生—药学]