TNFα基因-863位点单核苷酸多态性与宫颈癌以及HPV感染的关系  

Association of-863 polymorphism of TNFα gene with cervical cancer and HPV infection

作　　者：尉春艳[1] 吴静[1] 张熙[2] 张菊[3] 高艳娥[1] 

机构地区：[1]西安交通大学医学院第二附属医院妇产科,陕西西安710004 [2]西安交通大学医学院第一附属医院神经外科,陕西西安710061 [3]第四军医大学基因诊断技术研究所,陕西西安710033

出　　处：《西安交通大学学报（医学版）》2009年第2期225-228,243,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的探讨TNFα基因-863位点的单核苷酸多态性与宫颈癌以及HPV感染的关系。方法采用模板介导的染料终止物掺入一荧光偏振检测（TDI-FP）的方法对59例宫颈癌以及22例对照进行TNFα基因一863位点的单核苷酸多态性检测。结果宫颈癌组的TNFα基因-863位点A等位基因频率（39．0％）较对照组（14．6％）明显升高（P〈0．01，OR=2．673，95％CI：1．300～5．497）；AA基因型在宫颈癌组和对照组中分别为15．3％和0，差异显著（P〈0．05）；CA基因型在两组中分别为47．5％和29．2％，差异显著（P〈O．05）；A等位基因频率在HPV阳性者与HPV阴性者之间没有显著性差异（P〉0．05，OR：1．950，95％CI：0．840～4．527），但是在HPV阳性者中具有增高趋势（37．5％），高于HPV阴性者（19．2％）。结论TNFα基因863位点A等位基因以及CA、AA基因型与宫颈癌的危险性升高有关，与HPV感染危险性无关。Objective To analyze the relationship between cervical cancer, and the polymorphism of tumor necrosis factor α （TNFα） gene at the site of -863. Methods TDI-FP was adopted to detect the polymorphism of TNFα gene at the site of -863 in 59 cases and 22 controls. Results The positive rate of A allele at -863 in group of cervical cancer （39.0 % ） was significantly higher than that in control group （14.6 % ） （P〈0.01, OR = 2. 673, 95% CI ： 1. 300- 5.497）. Genotypes of CA and AA were 47.5 % and 15.3 % in cases of cervical cancer, and significantly low rates of 29.2% and 0% in cases of control, respectively （P〈0. 05）. Although no significant difference （P〉0. 05, OR =1. 950, 95% CI： 0. 840-4. 527） was found between HPV positive and negative groups, a trend of high positive rate of A allele at -863 in HPV positive group was found. Conclusion The A allele, AA genotypes and CA genotypes at -863 of TNFα gene is associated significantly with increased risk of cervical cancer. Polymorphism at -863 does not attribute to HPV infection.

关 键 词：肿瘤坏死因子Α 宫颈肿瘤 人乳头瘤病毒 单核苷酸多态性 荧光偏振 

分 类 号：R711.74[医药卫生—妇产科学]