Polydentate cyclotriphosphazene ligands:Design,synthesis and bioactivity  

作　　者：Le Wang Yong Ye Shang Bin Zhong Yu Fen Zhao 

机构地区：[1]Key Laboratory of Chemical Biology and Organic Chemistry, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China [2]Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China

出　　处：《Chinese Chemical Letters》2009年第1期58-61,共4页中国化学快报（英文版）

基　　金：supports from the NNSFC(Nos.20602032,20732004 and 20572061).

摘　　要：Five multinuclear cyclotriphosphazene ligands were synthesized and tested for their cleavage activities to plasmid DNA. All of these new compounds were confirmed by MS, ^1H NMR, ^31P NMR, ^13C NMR and IR. Preliminary studies on the cleavage of pUC19 DNA in the presence of metal complexes were performed. The results revealed that these complexes could act as powerful catalysts under physiological conditions. The complexes 3b ＋ Cu can effectively cleave DNA to nicked form, giving hydrolysis rate constant of 0.08/h under physiological conditions. An acid-base catalyzed DNA phosphate-diester hydrolysis mechanism was also orooosed.Five multinuclear cyclotriphosphazene ligands were synthesized and tested for their cleavage activities to plasmid DNA. All of these new compounds were confirmed by MS, ^1H NMR, ^31P NMR, ^13C NMR and IR. Preliminary studies on the cleavage of pUC19 DNA in the presence of metal complexes were performed. The results revealed that these complexes could act as powerful catalysts under physiological conditions. The complexes 3b ＋ Cu can effectively cleave DNA to nicked form, giving hydrolysis rate constant of 0.08/h under physiological conditions. An acid-base catalyzed DNA phosphate-diester hydrolysis mechanism was also orooosed.

关 键 词：CYCLOTRIPHOSPHAZENE Polydentate ligand DNA cleavage HYDROLYSIS 

分 类 号：O641.4[理学—物理化学] S482.39[理学—化学]