Determination of ephedrine and codeine in human urine by cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography  

作　　者：Li Jun Li Si Guang Li Hai Yan Li Zhuo Cai Hao Cheng 

机构地区：[1]Department of Biological and Chemical Engineering, Guangxi University of Technology, Liuzhou 545006, China [2]College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China [3]Zhongshan School of Medicine, Sun-Yat-Sen University, Guangzhou 510080, China

出　　处：《Chinese Chemical Letters》2009年第1期84-87,共4页中国化学快报（英文版）

基　　金：supported by the Guangxi Provincial Foundation of Natural Science(No.0481019).

摘　　要：A sensitive method for the determination of ephedrine and codeine in human urine by capillary electrophoresis （CE） was described. In order to improve the sensitivity, two online concentration techniques including cation-selective exhaustive injection （CSEI） and sweeping micellar electrokinetic chromatography （sweeping-MEKC） were used. Under the optimum conditions, the detection limits （S/N = 3） were 0.10 μg/L for ephedrine and 0.80 μg/L for codeine. This method was successfully applied to real urine sample analysis.A sensitive method for the determination of ephedrine and codeine in human urine by capillary electrophoresis （CE） was described. In order to improve the sensitivity, two online concentration techniques including cation-selective exhaustive injection （CSEI） and sweeping micellar electrokinetic chromatography （sweeping-MEKC） were used. Under the optimum conditions, the detection limits （S/N = 3） were 0.10 μg/L for ephedrine and 0.80 μg/L for codeine. This method was successfully applied to real urine sample analysis.

关 键 词：Cation-selective exhaustive injection Sweeping miceUar electrokinetic chromatography EPHEDRINE CODEINE 

分 类 号：O657.8[理学—分析化学] TQ425.236[理学—化学]