藤黄酸抑制NF-κB信号通路阻滞Raji细胞周期进程  

Gambogic Acid Arrested Cell Cycle of Raji Cells through Inhibition of the NF-κB Signaling Pathway

作　　者：王勇[1,2] 陈燕 吴小建[2] 陈子[1] 柯文娟[1] 舒文秀[1,3] 吴秋玲[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液病研究所,武汉430022 [2]九江学院附属医院血液肿瘤科,九江332000 [3]宁波市医疗中心李惠利医院血液肿瘤科,宁波315000

出　　处：《华中科技大学学报（医学版）》2009年第2期169-172,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30472267)

摘　　要：目的探讨藤黄酸对人B细胞非霍奇金淋巴瘤(NHL)细胞系Raji细胞周期的影响及可能的信号通路。方法采用碘化丙啶染色流式细胞术检测细胞周期,免疫印迹检测Cyclin D3、Cyclin E和NF-κB蛋白表达。结果藤黄酸能明显抑制Raji细胞增殖,呈剂量依赖性阻滞Raji细胞周期于G0/G1期,下调Cyclin D3、Cyclin E和NF-κB蛋白表达。结论藤黄酸通过抑制调节Cyclin D3和Cyclin E的表达,阻滞细胞周期进程,该过程可能与NF-κB传导通路有关。Objective To explore the effect of Gambogic acid on the cell cycle of Raji cells and its underlying signaling pathway. Methods Cell cycle was examined by PI staining through flow eytometry. The expression of Cyelin D3,Cyelin E,and NF-κB was detected by Western blot. Results In a dose-dependent manner,Gambogic acid arrested cell cycle of Raji cells in G0/ G1 phase,and downregulated the expression of Cyclin D3,Cyclin E,and NF-κB. Conclusion Gambogic acid arrested cell cycle of Raji cells through inhibition of the NF-κB signaling pathway.

关 键 词：藤黄酸 细胞周期 核因子-ΚB 细胞周期蛋白D3 细胞周期蛋白E 

分 类 号：R349.5[医药卫生—基础医学]