PRGD/PDLLA/β-TCP/NGF复合神经导管的制备及其体外降解性能  

Preparation and degradation performance of PRGD/PDLLA/β-TCP/NGF composite nerve conduit

作　　者：王永红[1] 戴红莲[2] 严琼姣[2] 李世普[2] 

机构地区：[1]武汉理工大学医院,湖北武汉430070 [2]武汉理工大学生物材料与工程研究中心,湖北武汉430070

出　　处：《中南大学学报（自然科学版）》2009年第2期406-410,共5页Journal of Central South University:Science and Technology

基　　金：国家"973"重点基础研究发展计划资助项目(G2005CB623905)

摘　　要：将聚合物-RGD多肽接枝聚(乳酸-羟基乙酸L-赖氨酸,PRGD)与聚(D,L-乳酸,PDLLA)、β-磷酸三钙(β-TCP)、神经生长因子(NGF)复合制备的新型PRGD/PDLLA/β-TCP/NGF复合导管材料在37℃的磷酸缓冲溶液中进行降解试验,研究支架材料在体外降解过程中相对分子质量、质量、降解介质pH值、微观形貌的变化及NGF的释放规律。研究结果表明:NGF的释放量在30d内可以达到诱导神经组织的功能;加入β-TCP,可以部分中和聚乳酸和PRGD降解产物的酸性,有效调节降解介质的pH值,使其维持在中性。加入PRGD和NGF可促进复合膜的降解,PRGD/PDLLA/β-TCP/NGF复合材料比聚乳酸具有更好的降解性能。The PRGD/PDLLA/β-TCP/NGF composite nerve conduits were prepared by solvent evaporation method using poly {（lactic acid）-co-[（glycolic acid）-alt-（L-lysine）]}（PRGD）, poly （D,L-lactide）（PDLLA）, β-tricalcium（β-TCP） and nerve growth factor（NGF）. The degradation performance of composite nerve conduits were studied by observing the variation of pH value of degradation medium and relative molecular mass, mass loss ratio, NGF releasing quantity and microstructure of the composite. The results show that the releasing quantity of NGF can achieve the function inducing nerve tissue growth within 30d; β-TCP can neutralize the acidity produced by degradation of PDLLA and PRGD, availably adjusts pH value of degradation medium to keep at neuter. Adding PRGD and β-TCP can promote the composite to degrade, hence, PRGD/PDLLA/β-TCP/NGF composite has better biodegradation than PDLLA.

关 键 词：RGD多肽接枝 聚(D L-乳酸) Β-磷酸三钙 神经生长因子 神经导管 降解 

分 类 号：R318.08[医药卫生—生物医学工程]