高胆红素血症对豚鼠畸变产物耳声发射、听性脑干反应和失匹配负波的影响  

Effects of hyperbilirubinemia on distortion product otoacoustic emission,auditory brainstem response and mismatch negativity in guinea pigs

作　　者：周昕[1] 梁勇[1] 李琦[1] 欧阳天斌[1] 谭曼玲[1] 

机构地区：[1]南方医科大学南方医院耳鼻咽喉头颈外科,广东广州510515

出　　处：《南方医科大学学报》2009年第4期768-771,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(8151051501000044)

摘　　要：目的探讨在高胆红素血症状态下豚鼠畸变产物耳声发射(DPOAE)、听性脑干反应(ABR)和失匹配负波(MMN)的反应特点,比较不同程度高胆红素血症对听觉功能的影响。方法选择正常幼年豚鼠30只,随机分成对照组、低剂量组和高剂量组,在建立高胆红素血症豚鼠模型基础上,检测其DPOAE、ABR及MMN,比较各组间不同检测结果的差异。结果各组间DPOAE检测通过率差异无统计学意义(P>0.05);ABR检测时,低剂量组仅Ⅲ、Ⅴ潜伏期和Ⅰ-Ⅲ波间期明显延长,较对照组差异有统计学意义(P<0.05),高剂量组各项指标较对照组差异均有统计学意义(P<0.05);各组间MMN检出率差异有统计学意义(χ2=7.438,P=0.024),高剂量组MMN潜伏期明显较对照组延长,差异有统计学意义(P<0.05),而MMN振幅差异无显著统计学意义(P>0.05)。结论高胆红素血症主要导致蜗后听觉传导通路的损害,对耳蜗损伤较为轻微;不仅引起感音神经性听觉功能障碍,还可能导致听觉中枢对声学信息处理出现异常。Objective To explore the characteristics of distortion product otoacoustic emission （DPOAE）, auditorybrainstem response （ABR） and mismatch negativity （MMN） in guinea pigs with hyperbilirubinemia and assess the impact of hyperbilirubinemia on their hearing functions. Methods Normal neonatal guinea pigs were randomly divided into normal control, low- and high-dose bilirubin groups. DPOAE, ABR and MMN were tested and compared between the 3 groups of guinea pigs. Results The DPOAE was comparable between the 3 groups （P〉0.05）. The guinea pigs with peritoneal injection of low-dose bilirubin exhibited significantly prolonged wave Ⅲ and V latencies and I-Ⅲ interwave intervals during the ABR test in comparison with the control group （P〈0.05）. All the ABR parameters were significantly different between the high-dose bilirubin group and the control group （p〈0.05）. The detection rates of MMN-were significantly different between the 3 groups （X^2=-7.438, P=-0.024）, and the average MMN latency was significantly shorter in the control group than in the high-dose group （P〈0.05）, but the amplitudes of MMN remained similar between the 3 groups （P〉0.05）. Conclusion Hyperbilirubinemia results impairment primarily of retrocoehlear auditory pathway with relative mild damage of the cochlear. Apart from sensorineural auditory impairment,hyperbilirubinemia may also cause central auditory processing abnormalities.

关 键 词：高胆红素血症 耳声发射 听性脑干反应 失匹配负波 豚鼠 

分 类 号：R722.17[医药卫生—儿科]