胶原蛋白多肽-铬(Ⅲ)螯合物提高糖尿病小鼠免疫功能的研究  

Effect of Collagen Peptide-Chromium(Ⅲ) Complex on Enhancing Diabetes Mice Immunity

作　　者：张国蓉[1] 张旭[1] 张程[1] 刘安军[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《现代食品科技》2009年第4期358-361,共4页Modern Food Science and Technology

基　　金：天津市应用基础及前沿技术研究计划重点项目(2009年)

摘　　要：研究了胶原蛋白多肽-铬(Ⅲ)(CPCC)螯合物提高糖尿病小鼠免疫功能的机制。通过腹腔注射四氧嘧啶造小鼠糖尿病模型,观察胶原蛋白多肽-铬(Ⅲ)螯合物对小鼠免疫器官的影响,血细胞染色观察中性粒细胞,用单项琼脂扩散免疫试验测定小鼠抗体,用酶联免疫法测定小鼠外周血细胞因子IL-2、IL-6,TNF-α等指标。结果表明,胶原蛋白多肽-铬(Ⅲ)螯合物可以促进小鼠分泌的IgG抗体明显增加,增强细胞因子IL-2和TNF-α的分泌,降低IL-6的分泌。胶原蛋白多肽-铬(Ⅲ)螯合物能活化免疫细胞,恢复和改善小鼠机体的免疫功能,提高小鼠机体的免疫力,一定程度地减轻四氧嘧啶的毒性作用,降低血糖。To study the mechanism of enhancement of alloxan-induced diabetes mouse immunity by collagen peptide-chromium（Ⅲ） complex （CPCC） , Diabetes mouse model was set up by injecting alloxan and theft neutrophile, antibody and cytokine （IL-2, IL-6 and TNF-α） of peripheral blood were detected by blood cells staining, single immunodiffusion test, and ELISA method, respectively. Results showed that Collagen peptide-chromium（Ⅲ） complex could markedly stimulate the secretion of mice IgG antibody, enhance secretion of IL-2 and TNF-α cytokine, decrease the secretion of IL-6. It was concluded that Collagen peptide-chromium（Ⅲ） complex could activate immune cell, restore and improve mouse immoral function, enhance mouse immunity, reduce the toxic effect of alloxan in certain extent, then reduce the blood glucose.

关 键 词：胶原蛋白多肽-铬(Ⅲ)螯合物 四氧嘧啶 HE染色 单向琼脂扩散 

分 类 号：R587.1[医药卫生—内分泌]