冠心病患者MBL基因启动子区-221位点多态性研究  

Study on Polymorphism in Promoter Region(-221 Site) of Mannose Binding Lectin Gene in Patients with Coronary Heart Disease

作　　者：周艳[1] 程健君[1] 刘迎春[2] 唐玉红[1] 贾天军[1] 韩小艳[3] 李小俊[3] 

机构地区：[1]河北北方学院医学技术学院免疫学教研室,河北张家口075000 [2]河北建筑工程学院校医院,河北张家口075000 [3]河北北方学院医学技术学院微生物学教研室,河北张家口075000

出　　处：《河北北方学院学报（医学版）》2009年第2期17-19,共3页Journal of Hebei North University:Medical Edition

基　　金：河北省自然科学基金资助项目(编号:C2006000875);863博士基金项目资助(编号:2003AA2Z3509)

摘　　要：目的:研究冠心病患者MBL基因启动子区-221(Y/X)位点单核苷酸多态性,探讨冠心病的可能致病机制。方法:提取57例健康对照者和72例冠心病患者外周血基因DNA,采用PCR-RFLP法检测MBL基因启动子区-221位点的多态性,行非变性的聚丙烯酰胺凝胶电泳对该位点多态性进行分析。结果:72例冠心病患者MBL基因启动子区-221位点X/Y、Y/Y和X/X基因型分别占69.44%、15.28%和15.28%;57例健康对照者在-221位点X/Y、Y/Y和X/X基因型分别占77.19%、1.75%和21.05%。经统计学分析,冠心病组、健康对照组在-221位点X/X和X/Y基因型频率比较差别无统计学意义;Y/Y基因型频率比较差别有显著性统计学意义(P<0.05)。结论:MBL基因启动子区-221位点的多态性可能参与了冠心病的发生、发展过程。Objective： To study the single nucleotide polymorphism in promoter region （-221 site）of mannose binding lectin（MBL）gene in patients with coronary heart disease（CHD）, try to analyze the possible mechanism of CHD. Methods：DNA was extracted from the leukocyte cell of each blood both in 57 control subjects and 72 CHD cases. PCR-RFLP was used to test the promoter regions（-221 site）of MBL gene; and run polyacrylamide gel（PAGE）in order to analyze the polymorphism in -221 site. Results：The promoter regions（- 221 site）of MBL gene X/Y,Y/Y,X/X genotype of the CHD were 69.44% 15.28% and 15.28% ,and that of control subjects were respectty 77.19% 1.75 % and 21.05 %. There was no statistic difference in X/X, X/Y genotype between these two groups（P〉0.05）;The frequency of Y/Y in CHD cases was higher than that of control（P〈0.05）. Conclusion：The polymorphism in promoter region at -221 site of MBL gene perhaps partici- pate in the generation and development of coronary heart disease.

关 键 词：冠状动脉疾病 甘露聚糖结合凝集素 聚合酶链反应 基因 启动区(遗传学) 

分 类 号：R392[医药卫生—免疫学]