携Sialyl Lewis^X超声微泡靶向探查缺血心肌的炎症分子“印记”  

Targeted detection of inflammatory‘imprint' in ischemic myocardium by ultrasound microbubbles with sialyl lewis^X

作　　者：燕翼[1] 李军华[1] 杨莉[2] 吴爵非[1] 纪丽景[1] 王鹏[1] 谢佳佳[1] 宾建平[1] 

机构地区：[1]南方医科大学南方医院心内科,广州市510515 [2]南方医科大学南方医院药学部,广州市510515

出　　处：《临床超声医学杂志》2009年第4期223-227,共5页Journal of Clinical Ultrasound in Medicine

基　　金：国家863计划项目(2006AA02Z478)

摘　　要：目的探讨应用靶向超声分子成像探查心肌缺血再灌注损伤炎症"印记"的可行性。方法采用"亲和素-生物素"桥接法构建携唾液酸化路易斯(Sialyl Lewis^X)靶向超声微泡(MB_(sLex))和同型对照微泡(MBc)。10只心肌缺血再灌注小鼠随机先后注入MB_(sLex)和MBc(间隔30 min),分别于注入5 min后行心肌对比超声检查,测量心肌缺血区和非缺血区的声强度(Ⅵ)。结果对比超声图像显示MB_(sLex)组缺血区心肌造影见显著增强,Ⅵ值高达23.52±1.08,而在MBc组缺血区心肌造影仅见轻度增强,Ⅵ值为9.81±0.41,两者之间差异有统计学意义(P<0.05)。但无论MB_(sLex)组还是MBc组,缺血区心肌Ⅵ值均明显高于非缺血区心肌Ⅵ值(P<0.05)。两组非缺血区心肌之间Ⅵ值未见明显差异。结论应用携sLex超声微泡行对比超声能够靶向探查心肌缺血再灌注损伤的炎症"印记"。Objective To explore the feasibility of inflammatory ＂imprint＂ in myocardial ischemical reperfusion injury detected by targeted myocardial contrast eehocardiography （MCE）. Methods Targeted ultrasound microbubbles with sialyl Lewisx （sialyl Lewis^x, sLex） and isotype control microbubbles were conjugated by ＂avidin- biotin＂ bridge. 10 mice with myocardial ischemical repeffusion were injected with MBsLex and MBc randomly （ interval was 30 rain ）, then examined by myocardial contrast echocardiography （MCE） after 5 rain, the video intensity （Ⅵ） was determined. Results Contrast eehocardiography images showed ischemic region myocardium was enhanced obviously in MBsLex group, Ⅵ was （23.52 ± 1.08）, and iscbemic region myocardium was enhanced in group MBc with VI of （9.81 ± 0.41 ）, there was significant difference between the two groups（ P 〈 0.05）. The Ⅵ of ischemic region myocardium was obviously higher than that in non - isehemic myocardium （ P 〈 0.05 ）. There was no significant difference in Ⅵ between the two groups. Conclusion Molecular imaging of seleetin with targeted contrast echoeardiography is possible to detect myocardial ischemiareperfusion injury effectively.

关 键 词：心肌缺血 再灌注 炎症印记 靶向微泡 心肌对比超声 唾液酸化路易斯 

分 类 号：R542.2[医药卫生—心血管疾病] R454.3[医药卫生—内科学]