苯甲基磺酰氟对有机磷化合物诱导的迟发性神经毒性的影响  

Effect of phenylmethylsulfonyl fluoride on organophosphorus compounds-induced delayed neurotoxicuty

作　　者：朴丰源[1] 

机构地区：[1]大连医科大学劳动卫生与环境卫生学教研室,辽宁大连116044

出　　处：《大连医科大学学报》2009年第2期123-127,133,共6页Journal of Dalian Medical University

基　　金：国家自然科学基金(30771819);辽宁省教育厅重点实验室项目(2008S073)

摘　　要：[目的]为进一步解析苯甲基磺酰氟(PMSF)对有机磷化合物(OP)诱导的迟发性神经毒性(OPIDN)的影响提供依据。[方法]80只单冠白色leghorn种雌性成鸡被分成17组。除了30 mg/kg溴苯磷(leptophos)组和40 mg/kg三甲基苯基磷酸酯(TOCP)组为10只鸡外,其余组均为4只。OP染毒后各组鸡是否出现OPIDN症状要连续观察25 d,并对其临床症状的评分值进行分析比较。[结果]OP暴露后第25天,各组鸡的迟发性神经毒性症状观察结果显示,30 mg/kg leptophos组、30 mg/kg leptophos染毒后再给60 mg/kg PMSF组、40 mg/kg TOCP组和40 mg/kgTOCP染毒后再给60 mg/kg PMSF组的鸡出现了迟发性神经毒性症状,其临床症状的评分值分别为4.2、5.5、3.0和4.5。同时给予30 mg/kg leptophos和1 mg/kg PMSF组、40 mg/kg TOCP和1 mg/kg PMSF组鸡也出现了迟发性神经毒性症状,其临床症状的评分值分别为0.8和1.2,明显低于30 mg/kg leptophos组和40 mg/kg TOCP组(P<0.05或P<0.01)。5 mg/kg leptophos染毒后再投予30 mg/kg leptophos组和5 mg/kg TOCP染毒后再投予40 mg/kgTOCP组的鸡则出现较重的迟发性神经毒性症状,其评分值分别达6.0和4.0。[结论]OP染毒前给予PMSF能防止诱发OPIDN,后给予PMSF时能增强OP的OPIDN。同时给予PMSF和OP时是否诱发OPIDN,取决于PMSF和OP的暴露浓度比例。两次OP染毒之间给予PMSF时,同样能预防OP诱发OPIDN。[ Objective] To provide evidences for exploring further effect of phenylmethylsulfonyl fluoride （PMSF） on organophosphorus compounds- induced delayed neurotoxicuty （OPIDN）. [ Methods ] Eighty chickens were divided into 17 groups. Ten chickens were given in the group received 30 mg/kg leptophos or 40 mg/kg TOCP and 4 chickens were given in each of the other groups. The clinical signs of OPIDN were observed for 25 days after OP administration and the clinical sign scores were analyzed. [ Results ] On 25 days after OP administration, the clinical signs of OPIDN were observed and their signs were 4.2, 5.5, 3.0, 4.5, respectively, in the groups received 30 mg/kg leptophos, 60 mg/kg PMSF after 30 mg/kh leptophos dosing, 40 mg/kg TOCP and 60 mg/kg PMSF after 40 mg/kg TOCP dosing. The clinical signs of OPIDN were also found in the group received 30 mg/kg leptophos with 1 mg/kg PMSF or 40 mg/kg TOCP with 1 mg/kg PMSF. Their signs were 0. 8 and 1.2, and significantly lower than that in the group received 30 mg/kg leptophos or 40 mg/kg TOCP. The more severe clinical signs of OPIDN were shown in the groups received 30 mg/kg leptophos after 5 mg/kg administration and 40 mg/kg TOCP after 5 mg/kg administration and their clinical sign scores were 6.0 and 4.0, respectively. [ Conclusions] It indicates that OPIDN can be prevented by pretreatment of PMSF or enhanced by posttreatment of PMSF. It also suggested that when OP was coadministered with PMSF, whether OPIDN is induced depends mainly on exposure ratio of . OP and PMSF. PMSF, when was administered between the two administrations of OP, can prevent frnm OPIDN

关 键 词：有机磷化合物 迟发性神经毒性 鸡 苯甲基磺酰氟 

分 类 号：R595.4[医药卫生—内科学]