胺碘酮对右房快速起搏兔肺静脉心肌袖细胞Kir3.1、KvLQT1、HERG基因表达的影响  

Effects of amiodarone on potassium channel gene expression in myocardial sleeves of pulmonary vein in rabbits with rapid atrial pacing

作　　者：王奖荣[1] 高梅[1] 刘蔚[1] 孙慧[2] 贾晓萌[2] 侯应龙[1] 

机构地区：[1]山东大学附属千佛山医院心内科,山东济南250014 [2]山东大学医学院,山东济南250012

出　　处：《中国心脏起搏与心电生理杂志》2009年第2期155-157,共3页Chinese Journal of Cardiac Pacing and Electrophysiology

基　　金：山东省自然科学基金资助项目(项目编号:-2005ZX03)

摘　　要：目的观察胺碘酮对右房快速起搏兔肺静脉心肌袖细胞膜钾通道亚型(Kir3.1、KvLQT1、HERG)基因表达的影响。方法新西兰兔30只,随机分为对照组、快速心房起搏组(起搏组,600次/分频率起搏7天)及心房快速起搏+胺碘酮组(胺碘酮组,快速起搏+胺碘酮灌胃7天),每组10只。剪取3组兔肺静脉心肌袖组织,应用逆转录-聚合酶链反应技术测定肺静脉心肌袖细胞Kir3.1、KvLQT1、HERG的mRNA表达水平。结果起搏组Kir3.1mRNA的表达低于对照组42.8%(P<0.01),胺碘酮组低于对照组46.4%(P<0.01);HERG mRNA的表达起搏组与对照组无差异(P>0.05),胺碘酮组比对照组高28.8%(P<0.05);KvLQT1三组无差异。结论快速心房起搏可引起兔肺静脉心肌袖细胞钾通道基因表达变化,胺碘酮对其亦有影响。Objective To investigate effects of amiodarone on potassium channel gene expression in myocardial sleeves of pulmonary vein in rabbits with rapid atrial pacing. Methods Rabbits were divided into control groups（ n = 10）, rapid atrial pacing（RAP） group（ RAP group, n = 10） and RAP and amiodarone group（ Am group, n = 10）. Myocardium tissues were obtained from rabbit puhnonary veins, the messenger ribonucleic acid （mRNA） of gir3.1, KvLQT1, HERG of specimen was measured by reverse transcription-polymerase chain reaction. Results Compared with the control group, Kir3.1 mRNA expression reduced by 42.8% in RAP group （P 〈0.01 ）, and 46.4% in Am group（P 〈0.01 ）. The mRNA levels of HERG significantly increased by 28.8% in Am group （P 〈 0.01 ） compared with the control group. There was no significant difference in mRNA levels of KvLQT1 among the three groups. Conclusion The RAP results in changes of the potassium channel gene expression in myocardial sleeves of pulmonary vein in rabbits and amiodarone also has some effect on them.

关 键 词：心血管病学 心房颤动 快速心房起搏 钾通道 胺碘酮 基因表达 

分 类 号：R541.75[医药卫生—心血管疾病] R318.11[医药卫生—内科学]