3种石斛兰种子非共生萌发和离体保存  

Non-symbiotic Germination of Three Species in Dendrobium and In vitro Conservation at Low Temperature

作　　者：余乐[1] 兰芹英[2] 王晓静[2] 马双敏[3] 梁国鲁[1] 

机构地区：[1]西南大学园艺园林学院,重庆北碚400715 [2]中国科学院西双版纳热带植物园 [3]云南大学生命科学学院

出　　处：《安徽农学通报》2009年第8期27-28,69,共3页Anhui Agricultural Science Bulletin

基　　金：国家自然科学基金(30571526);中国科学院战略生物资源技术支撑体系专项项目(CZBZX-1);科学技术部平台项目(2005DKA21006)

摘　　要：以金钗石斛(Dendrobium nobile)、流苏石斛(D.fimbriatum)和报春石斛(D.primulinum)为研究对象,研究了其在激素浓度配比下种子萌发情况,并对流苏石斛幼苗进行离体保存。结果表明:25℃时,KnudsonC(KC)+BA2mg/L+NAA0.2mg/L最利于金钗和流苏石斛原球茎诱导和幼苗分化;KC+NAA0.5mg/L时最适报春石斛原球茎诱导和幼苗分化。幼苗保存在15℃,每天12h光照(2000lx),添加3%甘露醇(mannitol)的KC培养基中,10个月后存活率为96%。Effect of different hormones combinations on the seed non-symbiotic germination of D. nobile, D. fimbriatum and D. primulinum also the in vitro conservation of D. fimbriatum seedings were studied in this paper. The results showed that KC＋ BA2mg/L ＋NAA0.2mg/Lat 25℃ was the best for D. nobile and D.fimbriatum protcorm growth and organogenesis; KC＋ NAA0.5mg/L at 25 ℃ was suitable for D. primulinum. Addition of mannitol （ 3.0%w/v ） to the medium and incubation at low temperature （ 15 ℃ ） resulted in higher survival rate （ 96% ） after 10 months of continuous storage.

关 键 词：石斛属 种子 非共生萌发 离体保存 

分 类 号：S682.31[农业科学—观赏园艺]