ZnO-MWCNTs/Nafion inorganic-organic composite film:Preparation and application in bioelectrochemistry of hemoglobin  

作　　者：Wei Ma Wei Song Dan Bi Tian 

机构地区：[1]College of Science, Nanjing University of Technology, Nanjing 210009, China

出　　处：《Chinese Chemical Letters》2009年第3期358-361,共4页中国化学快报（英文版）

摘　　要：Multi-walled carbon nanotubes （MWCNTs） were coated with ZnO by a hydrothermal method. The resulting nanocomposites were mixed with the Nation solution to form a composite matrix for the fabrication of hemoglobin （Hb） biosensor. To prevent the leak of Hb molecules of the biosensor, silica sol-gel film was coated on the surface of the Hb/ZnO-MWCNTs/Nafion electrode. The silica sol-gel/Hb/ZnO-MWCNTs/Nafion film exhibited a pair of well-defined, quasi-reversible redox peaks. This biosensor showed excellent electrocataiytic activity to H2O2. The sensitivity and apparent Michaelis-Menten constant of this Hb biosensor to H2O2 were 1.31 A/（M cm^2） and 82.8 μmol/L, respectively, which indicated that Hb had high affinity to H2O2.Multi-walled carbon nanotubes （MWCNTs） were coated with ZnO by a hydrothermal method. The resulting nanocomposites were mixed with the Nation solution to form a composite matrix for the fabrication of hemoglobin （Hb） biosensor. To prevent the leak of Hb molecules of the biosensor, silica sol-gel film was coated on the surface of the Hb/ZnO-MWCNTs/Nafion electrode. The silica sol-gel/Hb/ZnO-MWCNTs/Nafion film exhibited a pair of well-defined, quasi-reversible redox peaks. This biosensor showed excellent electrocataiytic activity to H2O2. The sensitivity and apparent Michaelis-Menten constant of this Hb biosensor to H2O2 were 1.31 A/（M cm^2） and 82.8 μmol/L, respectively, which indicated that Hb had high affinity to H2O2.

关 键 词：HEMOGLOBIN ZNO Multi-walled carbon nanotubes Nation BIOELECTROCHEMISTRY 

分 类 号：TQ153.15[化学工程—电化学工业] Q51[生物学—生物化学]