红花木AFLP指纹图谱的构建  

AFLP Based Fingerprint Map Establishment of Loropetalum Chinense Var.Rubrum

作　　者：李达[1] 于晓英[1] 熊兴耀[1] 彭尽晖[1] 吕长平[1] 张宏志[1] 陈海霞[1] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128

出　　处：《经济林研究》2009年第1期17-22,共6页Non-wood Forest Research

基　　金：湖南省自然基金项目"红花木种质资源的收集及AFLP鉴别与分类研究"(04JJ3024);湖南省教育厅省高等学校产业化培育项目"红花木新品种繁育及生产基地建设"(07CY001)

摘　　要：为了获得红花木的清晰AFLP指纹图谱,以进一步研究红花木种质资源的鉴别与分类问题,以红花木的嫩叶、成熟叶、果实为材料,采用改进后的CTAB+Tris-HCl洗涤法、CTAB法、SDS+Tris-HCl洗涤法、SDS法,获得了HMW基因组DNA,其中以CTAB+Tris-HCl洗涤法提取的成熟叶片的DNA的纯度和浓度都很高,且获得了清晰的AFLP指纹图谱,这为AFLP技术在红花木种质资源的研究与应用奠定了基础。In order to obtain clear amplified fragment length polymorphism fingerprint map of Loropetalum chinense var. rubrum for identification and classification of its germsplasm resources, spires, mature leaves and fruits of Loropetalum chinense vat. rubrum were taken as materials, high molecular weight （HMW） genomic DNA were obtained using improved methods of CTAB with Tris-HC1 clean, CTAB, SDS with Tris-HC1 clean, SDS. The research got a clear fingerprint map of AFLP, and built the foundation for application of AFLP technology in study of Loropetalum chinense var. rubrum germsplasm resources.

关 键 词：红花木 AFLP 模板DNA 

分 类 号：Q789[生物学—分子生物学]