淋巴细胞亚群功能状态的影响  

Effects of Astragulus injection on Th cell subset function in patients with psoriasis in vitro

作　　者：吴媛[1] 冯文莉[1] 张荣丽[1] 

机构地区：[1]山西医科大学第二医院

出　　处：《临床医药实践》2009年第4期258-260,共3页Proceeding of Clinical Medicine

基　　金：山西省青年科技研究基金资助项目(课题编号:20011030)

摘　　要：目的：探讨黄芪注射液对银屑病患者辅助性T淋巴细胞（Th细胞）亚群功能状态的影响。方法：对20例银屑病患者的外周血淋巴细胞体外分别经植物血凝素（PHA）和PHA＋黄芪诱导：即病例对照组和病例黄芪组，同时对10例健康志愿者外周血淋巴细胞体外经PHA诱导（即正常对照组），各组培养48h，用ELISA法检测培养上清液中Th类细胞因子IFN-γ和IL-4含量。结果：病例对照组IFN-γ水平高于正常对照组（P〈0.05），而IL-4水平低于正常对照组（P〈0．05）。病例黄芪组IFN-γ水平低于病例对照组（P〈0．05），而IL-4水平高于病例对照组（P〈0．05）。结论：黄芪注射液可显著下调银屑病患者Th1亚群优势状态。促进向Th2亚群转换，改善患者Th1／Th2失衡状态，对银屑病的治疗具有重要意义。Objective：To study the effects of Astragulus injection on Th cell subset function in patients with psoriasis. Methods ：The peripheral blood lymphocytes from 20 patients with psoriasis at the remission stage were stimulated with either phytohem agalutinin （PHA）（patient-control group）or PHA together with Astragulus （patient-Astragulus group）and were then cultured in vitro for 48 hours. The samples from 10 healthy people stimulated with PHA were used as the normal control group. The levels of interferon-γ（IFN-γ） and interleuikin-4（IL-4） in the supernatants of lymphocytes were detected using ELISA. Results：The IFN-γ level in the patient-control group were statistically higher than those in the control group（P〈0.05）. But the IL-4 lever were lower than those（P〈0.05）, The level of IFN-γ in the patient-Astragulus group were markedly lower than those in the patient-control group（P〈 0.05）. But the IL-4 level were higher than those（P〈0.05）. Conclusion：Astragulus injection can lower the Th1 cell subset and promote disverting to Th2 cell subset,therefor shows an importont significance in treating psoriasis.

关 键 词：黄芪注射液 银屑病 TH细胞亚群 IFN-Γ IL-4 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]