IL-18增强日本血吸虫DNA疫苗Sj23的免疫保护效果(英文)  

IL-18 augments protective immunity of Sj23 plasmid DNA vaccine against Schistosoma japonicum in mice

作　　者：门静涛[1,2] 刘全[2] 商利民[2] 林矫骄[2,3] 傅志强[2,3] 石耀军[2,3] 夏志平[2] 张西臣[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]军事医学科学院军事兽医研究所,吉林长春130062 [3]中国农业科学院上海兽医研究所,上海200232

出　　处：《中国兽医学报》2009年第5期610-614,共5页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划资助项目(2006BAD06A09)~~

摘　　要：将小鼠IL-18基因和日本血吸虫Sj23膜蛋白基因分别插入pVAX1载体,构建真核表达质粒pVAX/ mIL-18和pVAX/Sj23 ,联合或单独免疫小鼠,以pVAX1空载体作对照,免疫2次,间隔2周,2次免疫后4周攻击日本血吸虫尾蚴。体液和细胞免疫检测结果表明,联合免疫组能诱导小鼠产生较强的抗日本血吸虫成虫可溶性抗原(SWAP)IgG,较高水平的IFN-γ和IL-2。攻虫试验表明,联合免疫小鼠成虫减虫率和肝脏减卵率分别达41 .6 %和49 .4 %,明显高于pVAX/Sj23单独免疫组(减虫率和肝脏减卵率分别为26 .5 %和41 .4 %)。以上试验结果表明,IL-18能明显增强Sj23 DNA疫苗在小鼠体内的免疫应答,并且产生较强的保护作用。Two recombinant plasmids pVAX/Sj23 and pVAX/mlL-18 containing Schistosoma japonicum 23 000 membrane protein （Sj23） and routine If.-18 were evaluated for their ability to induce immune responses and to protect against S. japonicum challenge in mice. All animals vaccinated with pVAX/Sj23 alone or plus pVAX/mIL-18 developed specific anti SWAP （soluble worm antigen preparation） ELISA antibody and splenocyte proliferation re- sponse,and co-injection of pVAX/mlL 18 significantly increased the production of IFN-y and IL-2 compared with pVAX/Sj23 alone, indicating that IL-18 enhances the Thl-dominant immune response. The challenge experiment showed that worm reduction rates in pVAX/Sj23 group compared with control group （pVAX1） was 26.5% and in the pVAX/Sj23 plus pVAX/mIL-18 group was 41.9% ,and the hepatic egg reduction rates were 42.7 and 49.6%, respectively. These results indicated that co-injection of an IL-18 plasmid with Sj23 DNA vaccine efficiently improves the protective effect against S. japonicum infection.

关 键 词：日本血吸虫 SJ23 IL-18 DNA疫苗 小鼠 

分 类 号：R535[医药卫生—内科学] S852.735[医药卫生—临床医学]