茯苓酸对SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞分泌P-选凝素、sICAM-1及TNF-α的影响  

Effects of various concentrations of pachymic acid against SLT-Ⅱe on P-selectin,sICAM-1 and TNF-α production in rat intestinal microvascular endothelial cells

作　　者：周宏超[1,2] 索占伟[2] 范光丽[1] 刘钟杰[3] 穆祥[2] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]北京农学院兽医学(中医药)北京市重点实验室,北京102206 [3]中国农业大学动物医学院,北京100094

出　　处：《中国兽医学报》2009年第5期628-631,636,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30671543);北京市自然科学基金资助项目(6021001);北京市属市管高等学校人才强教计划资助项目(BAHED)

摘　　要：通过检测茯苓酸对SLT-Ⅱe诱导大鼠肠黏膜微血管内皮细胞分泌P-选凝素、sICAM-1及TNF-α的浓度变化,探索茯苓主要成分茯苓酸对仔猪水肿病的疗效机制。将培养的肠黏膜微血管内皮细胞分为对照组、SLT-Ⅱe组、不同质量浓度茯苓酸处理组5个组,采用ELISA法测定了培养3,6,9,12h细胞上清液中P-选凝素、sICAM-1及TNF-α浓度的变化。结果表明,不同质量浓度茯苓酸处理组对SLT-Ⅱe诱导肠黏膜微血管内皮细胞过量分泌P-选凝素、TNF-α无明显的抑制作用;10mg/L茯苓酸处理组对SLT-Ⅱe诱导肠黏膜微血管内皮细胞sICAM-1的分泌具有明显的下调作用,使其分泌sICAM-1的浓度逐渐下降至正常水平。由此可见,茯苓酸通过抑制SLT-Ⅱe诱导肠黏膜微血管内皮细胞sICAM-1的过量分泌,阻止白细胞与内皮细胞之间的牢固黏附,防止过度炎症反应对机体的损害,以达到治疗疾病的目的。茯苓酸保护大鼠肠黏膜微血管内皮细胞损伤的模型剂量为10mg/L。The study observed that various concentrations of pachymic acid against SLT-Ⅱe induce P-selectin, sI- CAM-1 and TNF-α production in rat intestinal microvascular endothelial cells （RIMECs） to explore curative effect mechanism of Edema disease of swine. The RIMECs were divided into control group, SLT- Ⅱ e group（ 1 mg/L SLT- Ⅱe） ,test 1 group（1 mg/L SLT-Ⅱ＋k acid） and test 3 group（1 mg/I. SLT-Ⅱe＋1 mg/L pachymic acid）,test 2 group （1 mg/L SLT-Ⅱ e＋5 mg/L pachymic e ＋10 mg/L pachymic acid） to study that various concentrations of pachymic acid against SLT lIe induce P-selectin,sICAM-1 and TNF-α production in RIMECs by ELISA. The results showed that pachymic acid inhibited P-selectin, TNF-α production induced by SLT-Ⅱe in RIMECs unmarkedly, and 10 mg/L pachymic acid inhibited slCAM-1 production induced by SLT-Ⅱe in RIMECs markedly. Effects of pachymic acid on the inhibited slCAM-1 production prevent fastness adhesive of leucocyte and endothelial cells,and alleviate excessive inflammation response. The model concentrations of pachymic acid was 10 mg/L to proteet the RIMECs.

关 键 词：志贺样毒素Ⅱ型变异体 肠黏膜微血管内皮细胞 P-选凝素 可溶性细胞间黏附分子-1 肿瘤坏死因子-α 茯苓酸 

分 类 号：S853.7[农业科学—临床兽医学] S852.61[农业科学—兽医学]