秤锤树嫩枝扦插过程中几种酶活性变化研究  

A Study on Changes of Some Enzymes in the Soft-stem Cuttings of Sinojackia xylocarpa

作　　者：徐丽萍[1] 上官新晨[2] 喻方圆[3] 

机构地区：[1]紫琅职业技术学院,江苏南通226001 [2]江西农业大学,江西南昌330045 [3]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《江西农业大学学报》2009年第2期274-277,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：2008国家林业局林业科技成果推广计划项目;国家科技攻关项目(2002BA906A38)

摘　　要：对秤锤树嫩枝扦插过程中吲哚乙酸氧化酶(IAAO)、多酚氧化酶(PPO)、过氧化物酶(POD)3种酶的活性进行测定。结果表明:嫩枝插穗内IAAO、POD、PPO活性在扦插初期逐渐上升,第7 d上升到高峰,之后下降再上升,第21 d达到新高峰,然后下降。这些酶活性上升分别在愈伤组织形成、根原基诱导阶段,但是上升幅度不一样。ABT6处理能抑制插穗IAAO活性,提高POD、PPO活性。The activities of indoleacetic acid oxidase （IAAO） , polyphenol oxidase （PPO） and peroxidase （POD） of Sinojackia xylocarpa soft - stem cuttings were studied. The results showed that： the activities of the three enzymes increased and reached their first peak on the 7th day, then decreased slightly followed by an increase and reached their second peak on the 21th day , then decreased again. The activities of these enzymes increased during the callus formatting phase, the root - inducing phase. However, their changing scopes were not the same. ABT6 could inhibit the activity of IAAO, but increase the activities of POD, PPO.

关 键 词：秤锤树 扦插 酶活性 

分 类 号：S794.901[农业科学—林木遗传育种]