氮源对莱茵衣藻生长和产氢的影响  

EFFECTS OF EXTRODANARY NITROGEN SOURCE ON GROWTH AND HYDROGEN PRODUCTION OF Chlamydomonas reinhardtii

作　　者：王荣荣[1] 阎光宇[1] 王全喜[1] 吴双秀[1] 刘晓磊[1] 

机构地区：[1]上海师范大学生命与环境科学学院生物系,上海200234

出　　处：《太阳能学报》2009年第4期546-550,共5页Acta Energiae Solaris Sinica

基　　金：上海市科委浦江人才计划(06PJ14076);上海市教育委员会科研创新项目(08YE66);上海市教育委员会重点学科建设项目(J50401);上海师范大学细胞生物学校级重点学科(DLZ808);上海市科学技术委员会基础性研究重点项目(06JC4091)

摘　　要：实验通过向莱茵衣藻培养基中加入不同浓度的甘氨酸和尿素,观察它们对莱茵衣藻细胞数和叶绿素含量的影响,筛选生长状态最佳的甘氨酸和尿素浓度,进而检测它们对衣藻产氢量和耗氧量的影响。结果表明甘氨酸和尿素的添加量分别为2.0g/L和0.5g/L时,莱茵衣藻的细胞数和叶绿素含量最高,此时产氢量提高了2～16倍,该结果为利用衣藻产氢的研究奠定了实验基础。Chlamydomonas reinhardtii is a model species for study of H2 production. Its H2 evolution was affected by its growth condition and chlorophyll content, and the synthesis of chlorophyll was related to glyeine and urea. In our study, we added glycine and urea into cultures of Chlamydomonas reinhardtii with different concentrationsand and detected their effects on cell densities, chlorophyll contents, H2 evolution and 02 consumption under light and dark in order to optimize the concentration of glycine and urea for improvement of H2 production of Chlamydomonas reinhardtii. The highest cell density and chlorophyll content of Chlamydomonas reinhardtii was achieved when 2.0g·L^-1 glycine and 0.5g·L^-1 urea added in normal TAP medium and H2 production was improved for 2-16 times. Our results demonstrated that glyeine and urea could benefit the H2 production of Chlamydomonas reinhardtii in beth normal TAP medium and sulfur-free medium.

关 键 词：莱茵衣藻 生物制氢 甘氨酸 尿素 

分 类 号：TQ116.29[化学工程—无机化工]