HPLC法测定头花蓼及制剂热淋清颗粒中槲皮苷的含量  

Determination of quercitrin in Polygonum capitatum and Relinqing granules by HPLC

作　　者：谢宇[1] 张丽艳[1] 梁斌 李孟林 唐靖雯 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]贵州威门药业股份有限公司,贵州贵阳550018

出　　处：《中国中药杂志》2009年第8期984-986,共3页China Journal of Chinese Materia Medica

基　　金：黔科合重大专项(20086020)

摘　　要：目的：建立头花蓼及热淋清颗粒中槲皮苷的含量测定方法。方法：采用反相高效液相色谱法，Diamonsil^TM（钻石）C18柱（4．6mm×250mm，5μm），流动相甲醇-1％醋酸-四氢呋喃，流速1．0mL·min^-1，检测波长258nm，柱温25℃。结果：槲皮苷在0．082～0．408μg线性关系良好（r=0．99997），头花蓼平均回收率102．3％，RSD0．99％，热淋清颗粒平均回收率102．7％，RSD2．2％。结论：该法重复性好，专属性强，可用于控制头花蓼药材及其单方制剂热淋清颗粒的质量。为头花蓼药材规模化种植提供科学依据。Objective： To establish an HPLC method for determination the contents of quercitrin in Polygonum capitatum and Relinqing granules. Method： The samples were analyzed on a Diamonsil C18 colunm （4. 6 mm × 250 mm,5μm） eluted with the mo- bile phase consisted of methanol-1% HAc-THF. The flow rate was 1.0 mL.min^-1. The detection wavelength was set at 258 nm and the column temperature was 25 ℃. Result： The calibration curve was linear in the range of 0. 081 64-0. 408 4μg （r = 0. 999 97）. The average recovery rate of quercitrin in P. capitatum was 102. 3% （ RSD 0. 99% ） ,and was 102. 7% （ RSD 2.2% ） in Relinqing granules. Conclusion： The method is reliable and specific with good repeatability, and can be used for the quality control of P. capitatum and Relinqing granules. It can provide a science bases for the planting of polygonum capitatum.

关 键 词：头花蓼 热淋清颗粒 槲皮苷 高效液相色谱 含量测定 

分 类 号：R284[医药卫生—中药学] R286[医药卫生—中医学]