金银花不同干燥技术HPLC指纹图谱研究  

Comparison on HPLC fingerprint profiles of Lonicera japonica bud obtained by various dryness techniques

作　　者：熊艳[1] 高慧敏[1] 王智民[1] 朱晶晶[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2009年第8期1015-1017,共3页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2006BAI09B05-9);国家自然科学基金项目(30672601)

摘　　要：目的:对不同干燥技术所得金银花进行HPLC指纹图谱的比较研究。方法:Kromasil C18色谱柱(4.6 mm×250mm,5μm),乙腈-0.4%的磷酸水溶液梯度洗脱,检测波长238 nm,柱温30℃,流速1 mL.min-1。结果:建立了不同干燥技术处理的金银花高效液相指纹图谱,确定了17个共有峰,但是成分的相对含量有明显差异。结论:利用HPLC指纹图谱分析法可较全面的反映不同干燥技术处理的金银花间的差异。Objective： To investigate ingredient differences of Lonicera japonica bud processed by different dryness techniques. Method： The HPLC fingerprint was used to evaluate the quality of L. japonica by various dryness process. The analysis was performed on a Kromasil C18 （4. 6 mm ×250mm, 5 μm）. Acetonitril and water containing 0. 4% H3PO4 were used as mobile phases with gradient elution. The flow rate was set at 1.0 mL.min^- 1 , the column temperature was set at 30℃, and the detection wavelength was at 238 nm. Result： The HPLC-fingerprint profiles of L. japonica from six different dryness techniques were determined. A total of 17 common peaks were found, but their contents from various dryness techniques were significantly different. Conclusion： HPLC-finger- print technique was one of potential Process Analytical Techniques （PAT） to explore the ingredient differences in dryness process.

关 键 词：金银花 干燥技术 绿原酸 木犀草苷 HPLC指纹图谱 

分 类 号：R284[医药卫生—中药学]