应用寡核苷酸探针检测甘薯基因组中NBS与LRR序列数目  

Estimation of copy number of NBS and LRR coding sequences in Ipomoea batatas genome by oligonucleotide probes

作　　者：屈满义[1] 查向东[1] 王钰[1] 杨金环[1] 蒋琳[1] 

机构地区：[1]安徽大学生命科学学院安徽省生态工程与生物技术重点实验室,合肥230039

出　　处：《生物学杂志》2009年第2期11-14,共4页Journal of Biology

基　　金：安徽省自然科学基金项目(050410104);安徽大学211工程学术创新团队资助(02203109)

摘　　要：以含编码NBS及LRR结构域序列的PCR扩增产物作标准对照,根据P-LOOP模体及LRR结构域氨基酸序列设计寡核苷酸探针,应用Southern杂交定量检测甘薯基因组中NBS与LRR序列的拷贝数目。结果表明,甘薯基因组中存在多个拷贝的NBS与LRR编码序列,经计算初步获得了其在基因组中的拷贝数目,为揭示甘薯抗病分子机制及植物抗病分子育种打下基础。The PCR amplified products containing NBS-LRR type sequence from sweet potato were used as the control samples. And southern blotting was performed to detect the copy numbers of NBS-LRR type sequences by the oligonueleotide probes designed according to the P-LOOP motif and LRR domain. The result showed that there existed multi-copies of NBS-LRR type sequence in Ipomoea batatas genome. The copy numbers of NBS-LRR type sequences were estimated. These results lay the foundation for revealing the molecular mechanism of disease-resistance of sweet potato and facilitate molecular breeding for disease resistance.

关 键 词：甘薯 NBS-LRR 寡核苷酸探针 SOUTHERN杂交 

分 类 号：Q943.2[生物学—植物学]